Genome sequence of Aedes aegypti, a major arbovirus vector

Vishvanath Nene; Jennifer R. Wortman; Daniel Lawson; Brian Haas; Chinnappa Kodira; Zhijian Tu; Brendan Loftus; Zhiyong Xi; Karyn Megy; Manfred Grabherr; Quinghu Ren; Evgeny M. Zdobnov; Neil F. Lobo; Kathryn S. Campbell; Susan E. Brown; Maria F. Bonaldo; Jingsong Zhu; Steven P. Sinkins; David G. Hogenkamp; Paolo Amedeo; Peter Arensburger; Peter W. Atkinson; Shelby Bidwell; Jim Biedler; Ewan Birney; Robert V. Bruggner; Javier Costas; Monique R. Coy; Jonathan Crabtree; Matt Crawford; Becky DeBruyn; David DeCaprio; Karin Eiglmeier; Eric Eisenstadt; Hamza El-Dorry; William M. Gelbart; Suely L. Gomes; Martin Hammond; Linda I. Hannick; James R. Hogan; Michael H. Holmes; David Jaffe; J. Spencer Johnston; Ryan C. Kennedy; Hean Koo; Saul Kravitz; Evgenia V. Kriventseva; David Kulp; Kurt LaButti; Eduardo Lee; Song Li; Diane D. Lovin; Chunhong Mao; Evan Mauceli; Carlos F.M. Menck; Jason R. Miller; Philip Montgomery; Akio Mori; Ana L. Nascimento; Horacio F. Naveira; Chad Nusbaum; Sinéad O'Leary; Joshua Orvis; Mihaela Pertea; Hadi Quesneville; Kyanne R. Reidenbach; Yu Hui Rogers; Charles W. Roth; Jennifer R. Schneider; Michael Schatz; Martin Shumway; Mario Stanke; Eric O. Stinson; Jose M.C. Tubio; Janice P. VanZee; Sergio Verjovski-Almeida; Doreen Werner; Owen White; Stefan Wyder; Qiandong Zeng; Qi Zhao; Yongmei Zhao; Catherine A. Hill; Alexander S. Raikhel; Marcelo B. Soares; Dennis L. Knudson; Norman H. Lee; James Galagan; Steven L. Salzberg; Ian T. Paulsen; George Dimopoulos; Frank H. Collins; Bruce Birren; Claire M. Fraser-Liggett; David W. Severson

doi:10.1126/science.1138878

Genome sequence of Aedes aegypti, a major arbovirus vector

Vishvanath Nene, Jennifer R. Wortman, Daniel Lawson, Brian Haas, Chinnappa Kodira, Zhijian Tu, Brendan Loftus, Zhiyong Xi, Karyn Megy, Manfred Grabherr, Quinghu Ren, Evgeny M. Zdobnov, Neil F. Lobo, Kathryn S. Campbell, Susan E. Brown, Maria F. Bonaldo, Jingsong Zhu, Steven P. Sinkins, David G. Hogenkamp, Paolo AmedeoPeter Arensburger, Peter W. Atkinson, Shelby Bidwell, Jim Biedler, Ewan Birney, Robert V. Bruggner, Javier Costas, Monique R. Coy, Jonathan Crabtree, Matt Crawford, Becky DeBruyn, David DeCaprio, Karin Eiglmeier, Eric Eisenstadt, Hamza El-Dorry, William M. Gelbart, Suely L. Gomes, Martin Hammond, Linda I. Hannick, James R. Hogan, Michael H. Holmes, David Jaffe, J. Spencer Johnston, Ryan C. Kennedy, Hean Koo, Saul Kravitz, Evgenia V. Kriventseva, David Kulp, Kurt LaButti, Eduardo Lee, Song Li, Diane D. Lovin, Chunhong Mao, Evan Mauceli, Carlos F.M. Menck, Jason R. Miller, Philip Montgomery, Akio Mori, Ana L. Nascimento, Horacio F. Naveira, Chad Nusbaum, Sinéad O'Leary, Joshua Orvis, Mihaela Pertea, Hadi Quesneville, Kyanne R. Reidenbach, Yu Hui Rogers, Charles W. Roth, Jennifer R. Schneider, Michael Schatz, Martin Shumway, Mario Stanke, Eric O. Stinson, Jose M.C. Tubio, Janice P. VanZee, Sergio Verjovski-Almeida, Doreen Werner, Owen White, Stefan Wyder, Qiandong Zeng, Qi Zhao, Yongmei Zhao, Catherine A. Hill, Alexander S. Raikhel, Marcelo B. Soares, Dennis L. Knudson, Norman H. Lee, James Galagan, Steven L. Salzberg, Ian T. Paulsen, George Dimopoulos, Frank H. Collins, Bruce Birren, Claire M. Fraser-Liggett, David W. Severson

Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

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Abstract

We present a draft sequence of the genome of Aedes aegypti, the primary vector for yellow fever and dengue fever, which at ∼1376 million base pairs is about 5 times the size of the genome of the malaria vector Anopheles gambiae. Nearly 50% of the Ae. aegypti genome consists of transposable elements. These contribute to a factor of ∼4 to 6 increase in average gene length and in sizes of intergenic regions relative to An. gambiae and Drosophila melanogaster. Nonetheless, chromosomal synteny is generally maintained among all three insects, although conservation of orthologous gene order is higher (by a factor of ∼2) between the mosquito species than between either of them and the fruit fly. An increase in genes encoding odorant binding, cytochrome P450, and cuticle domains relative to An. gambiae suggests that members of these protein families underpin some of the biological differences between the two mosquito species.

Original language	English (US)
Pages (from-to)	1718-1723
Number of pages	6
Journal	Science
Volume	316
Issue number	5832
DOIs	https://doi.org/10.1126/science.1138878
State	Published - Jun 22 2007

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Nene, V., Wortman, J. R., Lawson, D., Haas, B., Kodira, C., Tu, Z., Loftus, B., Xi, Z., Megy, K., Grabherr, M., Ren, Q., Zdobnov, E. M., Lobo, N. F., Campbell, K. S., Brown, S. E., Bonaldo, M. F., Zhu, J., Sinkins, S. P., Hogenkamp, D. G., ... Severson, D. W. (2007). Genome sequence of Aedes aegypti, a major arbovirus vector. Science, 316(5832), 1718-1723. https://doi.org/10.1126/science.1138878

Nene, V, Wortman, JR, Lawson, D, Haas, B, Kodira, C, Tu, Z, Loftus, B, Xi, Z, Megy, K, Grabherr, M, Ren, Q, Zdobnov, EM, Lobo, NF, Campbell, KS, Brown, SE, Bonaldo, MF, Zhu, J, Sinkins, SP, Hogenkamp, DG, Amedeo, P, Arensburger, P, Atkinson, PW, Bidwell, S, Biedler, J, Birney, E, Bruggner, RV, Costas, J, Coy, MR, Crabtree, J, Crawford, M, DeBruyn, B, DeCaprio, D, Eiglmeier, K, Eisenstadt, E, El-Dorry, H, Gelbart, WM, Gomes, SL, Hammond, M, Hannick, LI, Hogan, JR, Holmes, MH, Jaffe, D, Johnston, JS, Kennedy, RC, Koo, H, Kravitz, S, Kriventseva, EV, Kulp, D, LaButti, K, Lee, E, Li, S, Lovin, DD, Mao, C, Mauceli, E, Menck, CFM, Miller, JR, Montgomery, P, Mori, A, Nascimento, AL, Naveira, HF, Nusbaum, C, O'Leary, S, Orvis, J, Pertea, M, Quesneville, H, Reidenbach, KR, Rogers, YH, Roth, CW, Schneider, JR, Schatz, M, Shumway, M, Stanke, M, Stinson, EO, Tubio, JMC, VanZee, JP, Verjovski-Almeida, S, Werner, D, White, O, Wyder, S, Zeng, Q, Zhao, Q, Zhao, Y, Hill, CA, Raikhel, AS, Soares, MB, Knudson, DL, Lee, NH, Galagan, J, Salzberg, SL, Paulsen, IT, Dimopoulos, G, Collins, FH, Birren, B, Fraser-Liggett, CM & Severson, DW 2007, 'Genome sequence of Aedes aegypti, a major arbovirus vector', Science, vol. 316, no. 5832, pp. 1718-1723. https://doi.org/10.1126/science.1138878

@article{fcbc6ab7644340f18b97d5d7aed1cde8,

title = "Genome sequence of Aedes aegypti, a major arbovirus vector",

abstract = "We present a draft sequence of the genome of Aedes aegypti, the primary vector for yellow fever and dengue fever, which at ∼1376 million base pairs is about 5 times the size of the genome of the malaria vector Anopheles gambiae. Nearly 50% of the Ae. aegypti genome consists of transposable elements. These contribute to a factor of ∼4 to 6 increase in average gene length and in sizes of intergenic regions relative to An. gambiae and Drosophila melanogaster. Nonetheless, chromosomal synteny is generally maintained among all three insects, although conservation of orthologous gene order is higher (by a factor of ∼2) between the mosquito species than between either of them and the fruit fly. An increase in genes encoding odorant binding, cytochrome P450, and cuticle domains relative to An. gambiae suggests that members of these protein families underpin some of the biological differences between the two mosquito species.",

author = "Vishvanath Nene and Wortman, {Jennifer R.} and Daniel Lawson and Brian Haas and Chinnappa Kodira and Zhijian Tu and Brendan Loftus and Zhiyong Xi and Karyn Megy and Manfred Grabherr and Quinghu Ren and Zdobnov, {Evgeny M.} and Lobo, {Neil F.} and Campbell, {Kathryn S.} and Brown, {Susan E.} and Bonaldo, {Maria F.} and Jingsong Zhu and Sinkins, {Steven P.} and Hogenkamp, {David G.} and Paolo Amedeo and Peter Arensburger and Atkinson, {Peter W.} and Shelby Bidwell and Jim Biedler and Ewan Birney and Bruggner, {Robert V.} and Javier Costas and Coy, {Monique R.} and Jonathan Crabtree and Matt Crawford and Becky DeBruyn and David DeCaprio and Karin Eiglmeier and Eric Eisenstadt and Hamza El-Dorry and Gelbart, {William M.} and Gomes, {Suely L.} and Martin Hammond and Hannick, {Linda I.} and Hogan, {James R.} and Holmes, {Michael H.} and David Jaffe and Johnston, {J. Spencer} and Kennedy, {Ryan C.} and Hean Koo and Saul Kravitz and Kriventseva, {Evgenia V.} and David Kulp and Kurt LaButti and Eduardo Lee and Song Li and Lovin, {Diane D.} and Chunhong Mao and Evan Mauceli and Menck, {Carlos F.M.} and Miller, {Jason R.} and Philip Montgomery and Akio Mori and Nascimento, {Ana L.} and Naveira, {Horacio F.} and Chad Nusbaum and Sin{\'e}ad O'Leary and Joshua Orvis and Mihaela Pertea and Hadi Quesneville and Reidenbach, {Kyanne R.} and Rogers, {Yu Hui} and Roth, {Charles W.} and Schneider, {Jennifer R.} and Michael Schatz and Martin Shumway and Mario Stanke and Stinson, {Eric O.} and Tubio, {Jose M.C.} and VanZee, {Janice P.} and Sergio Verjovski-Almeida and Doreen Werner and Owen White and Stefan Wyder and Qiandong Zeng and Qi Zhao and Yongmei Zhao and Hill, {Catherine A.} and Raikhel, {Alexander S.} and Soares, {Marcelo B.} and Knudson, {Dennis L.} and Lee, {Norman H.} and James Galagan and Salzberg, {Steven L.} and Paulsen, {Ian T.} and George Dimopoulos and Collins, {Frank H.} and Bruce Birren and Fraser-Liggett, {Claire M.} and Severson, {David W.}",

year = "2007",

month = jun,

day = "22",

doi = "10.1126/science.1138878",

language = "English (US)",

volume = "316",

pages = "1718--1723",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "5832",

}

TY - JOUR

T1 - Genome sequence of Aedes aegypti, a major arbovirus vector

AU - Nene, Vishvanath

AU - Wortman, Jennifer R.

AU - Lawson, Daniel

AU - Haas, Brian

AU - Kodira, Chinnappa

AU - Tu, Zhijian

AU - Loftus, Brendan

AU - Xi, Zhiyong

AU - Megy, Karyn

AU - Grabherr, Manfred

AU - Ren, Quinghu

AU - Zdobnov, Evgeny M.

AU - Lobo, Neil F.

AU - Campbell, Kathryn S.

AU - Brown, Susan E.

AU - Bonaldo, Maria F.

AU - Zhu, Jingsong

AU - Sinkins, Steven P.

AU - Hogenkamp, David G.

AU - Amedeo, Paolo

AU - Arensburger, Peter

AU - Atkinson, Peter W.

AU - Bidwell, Shelby

AU - Biedler, Jim

AU - Birney, Ewan

AU - Bruggner, Robert V.

AU - Costas, Javier

AU - Coy, Monique R.

AU - Crabtree, Jonathan

AU - Crawford, Matt

AU - DeBruyn, Becky

AU - DeCaprio, David

AU - Eiglmeier, Karin

AU - Eisenstadt, Eric

AU - El-Dorry, Hamza

AU - Gelbart, William M.

AU - Gomes, Suely L.

AU - Hammond, Martin

AU - Hannick, Linda I.

AU - Hogan, James R.

AU - Holmes, Michael H.

AU - Jaffe, David

AU - Johnston, J. Spencer

AU - Kennedy, Ryan C.

AU - Koo, Hean

AU - Kravitz, Saul

AU - Kriventseva, Evgenia V.

AU - Kulp, David

AU - LaButti, Kurt

AU - Lee, Eduardo

AU - Li, Song

AU - Lovin, Diane D.

AU - Mao, Chunhong

AU - Mauceli, Evan

AU - Menck, Carlos F.M.

AU - Miller, Jason R.

AU - Montgomery, Philip

AU - Mori, Akio

AU - Nascimento, Ana L.

AU - Naveira, Horacio F.

AU - Nusbaum, Chad

AU - O'Leary, Sinéad

AU - Orvis, Joshua

AU - Pertea, Mihaela

AU - Quesneville, Hadi

AU - Reidenbach, Kyanne R.

AU - Rogers, Yu Hui

AU - Roth, Charles W.

AU - Schneider, Jennifer R.

AU - Schatz, Michael

AU - Shumway, Martin

AU - Stanke, Mario

AU - Stinson, Eric O.

AU - Tubio, Jose M.C.

AU - VanZee, Janice P.

AU - Verjovski-Almeida, Sergio

AU - Werner, Doreen

AU - White, Owen

AU - Wyder, Stefan

AU - Zeng, Qiandong

AU - Zhao, Qi

AU - Zhao, Yongmei

AU - Hill, Catherine A.

AU - Raikhel, Alexander S.

AU - Soares, Marcelo B.

AU - Knudson, Dennis L.

AU - Lee, Norman H.

AU - Galagan, James

AU - Salzberg, Steven L.

AU - Paulsen, Ian T.

AU - Dimopoulos, George

AU - Collins, Frank H.

AU - Birren, Bruce

AU - Fraser-Liggett, Claire M.

AU - Severson, David W.

PY - 2007/6/22

Y1 - 2007/6/22

N2 - We present a draft sequence of the genome of Aedes aegypti, the primary vector for yellow fever and dengue fever, which at ∼1376 million base pairs is about 5 times the size of the genome of the malaria vector Anopheles gambiae. Nearly 50% of the Ae. aegypti genome consists of transposable elements. These contribute to a factor of ∼4 to 6 increase in average gene length and in sizes of intergenic regions relative to An. gambiae and Drosophila melanogaster. Nonetheless, chromosomal synteny is generally maintained among all three insects, although conservation of orthologous gene order is higher (by a factor of ∼2) between the mosquito species than between either of them and the fruit fly. An increase in genes encoding odorant binding, cytochrome P450, and cuticle domains relative to An. gambiae suggests that members of these protein families underpin some of the biological differences between the two mosquito species.

AB - We present a draft sequence of the genome of Aedes aegypti, the primary vector for yellow fever and dengue fever, which at ∼1376 million base pairs is about 5 times the size of the genome of the malaria vector Anopheles gambiae. Nearly 50% of the Ae. aegypti genome consists of transposable elements. These contribute to a factor of ∼4 to 6 increase in average gene length and in sizes of intergenic regions relative to An. gambiae and Drosophila melanogaster. Nonetheless, chromosomal synteny is generally maintained among all three insects, although conservation of orthologous gene order is higher (by a factor of ∼2) between the mosquito species than between either of them and the fruit fly. An increase in genes encoding odorant binding, cytochrome P450, and cuticle domains relative to An. gambiae suggests that members of these protein families underpin some of the biological differences between the two mosquito species.

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U2 - 10.1126/science.1138878

DO - 10.1126/science.1138878

M3 - Article

C2 - 17510324

AN - SCOPUS:34250902354

SN - 0036-8075

VL - 316

SP - 1718

EP - 1723

JO - Science

JF - Science

IS - 5832

ER -

Genome sequence of Aedes aegypti, a major arbovirus vector

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