Genome and transcriptome of a pathogenic yeast, Candida nivariensis

Yunfan Fan; Andrew N. Gale; Anna Bailey; Kali Barnes; Kiersten Colotti; Michal Mass; Luke B. Morina; Bailey Robertson; Remy Schwab; Niki Tselepidakis; Winston Timp

doi:10.1093/g3journal/jkab137

Genome and transcriptome of a pathogenic yeast, Candida nivariensis

Yunfan Fan
, Andrew N. Gale
, Anna Bailey
, Kali Barnes
, Kiersten Colotti
, Michal Mass
, Luke B. Morina
, Bailey Robertson
, Remy Schwab
, Niki Tselepidakis
, Winston Timp

Whiting School of Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

We present a highly contiguous genome and transcriptome of the pathogenic yeast, Candida nivariensis. We sequenced both the DNA and RNA of this species using both the Oxford Nanopore Technologies and Illumina platforms. We assembled the genome into an 11.8 Mb draft composed of 16 contigs with an N50 of 886 Kb, including a circular mitochondrial sequence of 28 Kb. Using direct RNA nanopore sequencing and Illumina cDNA sequencing, we constructed an annotation of our new assembly, supplemented by lifting over genes from Saccharomyces cerevisiae and Candida glabrata.

Original language	English (US)
Article number	jkab137
Journal	G3: Genes, Genomes, Genetics
Volume	11
Issue number	7
DOIs	https://doi.org/10.1093/g3journal/jkab137
State	Published - Jul 2021

Keywords

Candida nivariensis
Genome assembly
Nanopore sequencing
Pathogenic yeast
Transcriptome

ASJC Scopus subject areas

Molecular Biology
Genetics
Genetics(clinical)

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10.1093/g3journal/jkab137

Cite this

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title = "Genome and transcriptome of a pathogenic yeast, Candida nivariensis",

abstract = "We present a highly contiguous genome and transcriptome of the pathogenic yeast, Candida nivariensis. We sequenced both the DNA and RNA of this species using both the Oxford Nanopore Technologies and Illumina platforms. We assembled the genome into an 11.8 Mb draft composed of 16 contigs with an N50 of 886 Kb, including a circular mitochondrial sequence of 28 Kb. Using direct RNA nanopore sequencing and Illumina cDNA sequencing, we constructed an annotation of our new assembly, supplemented by lifting over genes from Saccharomyces cerevisiae and Candida glabrata.",

keywords = "Candida nivariensis, Genome assembly, Nanopore sequencing, Pathogenic yeast, Transcriptome",

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T1 - Genome and transcriptome of a pathogenic yeast, Candida nivariensis

AU - Fan, Yunfan

AU - Gale, Andrew N.

AU - Bailey, Anna

AU - Barnes, Kali

AU - Colotti, Kiersten

AU - Mass, Michal

AU - Morina, Luke B.

AU - Robertson, Bailey

AU - Schwab, Remy

AU - Tselepidakis, Niki

AU - Timp, Winston

N1 - Publisher Copyright: © The Author(s) 2021. Published by Oxford University Press on behalf of Genetics Society of America.

PY - 2021/7

Y1 - 2021/7

N2 - We present a highly contiguous genome and transcriptome of the pathogenic yeast, Candida nivariensis. We sequenced both the DNA and RNA of this species using both the Oxford Nanopore Technologies and Illumina platforms. We assembled the genome into an 11.8 Mb draft composed of 16 contigs with an N50 of 886 Kb, including a circular mitochondrial sequence of 28 Kb. Using direct RNA nanopore sequencing and Illumina cDNA sequencing, we constructed an annotation of our new assembly, supplemented by lifting over genes from Saccharomyces cerevisiae and Candida glabrata.

AB - We present a highly contiguous genome and transcriptome of the pathogenic yeast, Candida nivariensis. We sequenced both the DNA and RNA of this species using both the Oxford Nanopore Technologies and Illumina platforms. We assembled the genome into an 11.8 Mb draft composed of 16 contigs with an N50 of 886 Kb, including a circular mitochondrial sequence of 28 Kb. Using direct RNA nanopore sequencing and Illumina cDNA sequencing, we constructed an annotation of our new assembly, supplemented by lifting over genes from Saccharomyces cerevisiae and Candida glabrata.

KW - Candida nivariensis

KW - Genome assembly

KW - Nanopore sequencing

KW - Pathogenic yeast

KW - Transcriptome

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M1 - jkab137

ER -

Genome and transcriptome of a pathogenic yeast, Candida nivariensis

Abstract

Keywords

ASJC Scopus subject areas

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