Genetic variation of capsule/LPS biogenesis in two serogroup O31 Vibrio cholerae isolates

Yuansha Chen; O. Colin Stine; J. Glenn Morris; Judith A. Johnson

doi:10.1111/j.1574-6968.2007.00808.x

Genetic variation of capsule/LPS biogenesis in two serogroup O31 Vibrio cholerae isolates

Yuansha Chen, O. Colin Stine, J. Glenn Morris, Judith A. Johnson

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Both NRT36S and A5 are NAG-ST-producing, serogroup O31 Vibrio cholerae. NRT36S is encapsulated and causes diarrhea when administered to volunteers; A5 is unencapsulated and does not colonize or cause illness in humans. The capsule/LPS (CPS/LPS) biogenesis regions in these two isolates were similar except that a 6.5-kb fragment in A5 has replaced a 10-kb fragment in NRT36S in the middle of the CPS/LPS gene cluster. Although the genes of the replaced region were homologous to genes from other CPS/LPS, they had little similarity to NRT36S and were not homologous to genes from other Vibrios. Data of this study highlight the apparent mobility within the CPS/LPS region that would provide a basis for the large number of observed V. cholerae serogroups and the emergence of novel epidemic strains.

Original language	English (US)
Pages (from-to)	133-139
Number of pages	7
Journal	FEMS Microbiology Letters
Volume	273
Issue number	2
DOIs	https://doi.org/10.1111/j.1574-6968.2007.00808.x
State	Published - Aug 2007
Externally published	Yes

Keywords

Gene transfer
Serogroup
Vibrio cholerae

ASJC Scopus subject areas

Genetics
Molecular Biology
Applied Microbiology and Biotechnology
Microbiology

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10.1111/j.1574-6968.2007.00808.x

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title = "Genetic variation of capsule/LPS biogenesis in two serogroup O31 Vibrio cholerae isolates",

abstract = "Both NRT36S and A5 are NAG-ST-producing, serogroup O31 Vibrio cholerae. NRT36S is encapsulated and causes diarrhea when administered to volunteers; A5 is unencapsulated and does not colonize or cause illness in humans. The capsule/LPS (CPS/LPS) biogenesis regions in these two isolates were similar except that a 6.5-kb fragment in A5 has replaced a 10-kb fragment in NRT36S in the middle of the CPS/LPS gene cluster. Although the genes of the replaced region were homologous to genes from other CPS/LPS, they had little similarity to NRT36S and were not homologous to genes from other Vibrios. Data of this study highlight the apparent mobility within the CPS/LPS region that would provide a basis for the large number of observed V. cholerae serogroups and the emergence of novel epidemic strains.",

keywords = "Gene transfer, Serogroup, Vibrio cholerae",

author = "Yuansha Chen and Stine, {O. Colin} and Morris, {J. Glenn} and Johnson, {Judith A.}",

year = "2007",

month = aug,

doi = "10.1111/j.1574-6968.2007.00808.x",

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journal = "FEMS Microbiology Letters",

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T1 - Genetic variation of capsule/LPS biogenesis in two serogroup O31 Vibrio cholerae isolates

AU - Chen, Yuansha

AU - Stine, O. Colin

AU - Morris, J. Glenn

AU - Johnson, Judith A.

PY - 2007/8

Y1 - 2007/8

N2 - Both NRT36S and A5 are NAG-ST-producing, serogroup O31 Vibrio cholerae. NRT36S is encapsulated and causes diarrhea when administered to volunteers; A5 is unencapsulated and does not colonize or cause illness in humans. The capsule/LPS (CPS/LPS) biogenesis regions in these two isolates were similar except that a 6.5-kb fragment in A5 has replaced a 10-kb fragment in NRT36S in the middle of the CPS/LPS gene cluster. Although the genes of the replaced region were homologous to genes from other CPS/LPS, they had little similarity to NRT36S and were not homologous to genes from other Vibrios. Data of this study highlight the apparent mobility within the CPS/LPS region that would provide a basis for the large number of observed V. cholerae serogroups and the emergence of novel epidemic strains.

AB - Both NRT36S and A5 are NAG-ST-producing, serogroup O31 Vibrio cholerae. NRT36S is encapsulated and causes diarrhea when administered to volunteers; A5 is unencapsulated and does not colonize or cause illness in humans. The capsule/LPS (CPS/LPS) biogenesis regions in these two isolates were similar except that a 6.5-kb fragment in A5 has replaced a 10-kb fragment in NRT36S in the middle of the CPS/LPS gene cluster. Although the genes of the replaced region were homologous to genes from other CPS/LPS, they had little similarity to NRT36S and were not homologous to genes from other Vibrios. Data of this study highlight the apparent mobility within the CPS/LPS region that would provide a basis for the large number of observed V. cholerae serogroups and the emergence of novel epidemic strains.

KW - Gene transfer

KW - Serogroup

KW - Vibrio cholerae

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ER -

Genetic variation of capsule/LPS biogenesis in two serogroup O31 Vibrio cholerae isolates

Abstract

Keywords

ASJC Scopus subject areas

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