TY - JOUR
T1 - Genetic relationships between bovine herpesvirus 4 and the gammaherpesviruses epstein-Barr virus and herpesvirus saimiri
AU - Bublot, Michel
AU - Lomonte, Patrick
AU - Lequarre, Anne Sophie
AU - Albrecht, Jens Christian
AU - Nicholas, John
AU - Fleckenstein, Bernhard
AU - Pastoret, Paul Pierre
AU - Thiry, Etienne
N1 - Funding Information:
We thank Marc Collet and Robert Herzog of the Free University of Brussels (Department of Molecular Biology) for their help in the computer analysis. We thank also Vicky van Santen and Gunther Keil for providing us their BHV-4 (DN 599 strain) sequences, Brigitte Bie-singer and Doris Biller their HVS sequences, and Marie-FranCoise Van Bressem its BHV4 (V. Test strain) sequences. The text presents research results of the Belgian National incentive program on fundamental research in life sciences initiated by the Belgian State, the Prime Minister’s Office Science Policy Program and was in part supported by the Deutsche Forschungsgemeinschaft, Forschergruppe “DNA-Viren des haematopoetischen Systems.” Scientific responsibility is assumed by the authors. We also thank Marinette Muys for the patient application of her secretarial skills.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1992/10
Y1 - 1992/10
N2 - The overall arrangement of genes in the unique central part of the bovine herpesvirus type 4 (BHV-4) genome has been deduced by analysis of short DNA sequences. Twenty-three genes conserved in at least one of the completely sequenced herpesviruses have been identified and localized. All of these genes encoded amino acid sequences with higher similarity to proteins of the gammaherpesviruses Epstein-Barr virus (EBV) and herpesvirus saimiri (HVS) than to the homologous products of the alphaherpesviruses varicella-zoster virus and herpes simplex virus type 1 or the betaherpesvirus human cytomegalovirus. The genome organization of BHV-4 had also an overall collinearity with that of the gammaherpesviruses EBV and HVS. Furthermore, the BHV-4 genes content and arrangement were more similar to those of HVS than to those of EBV, suggesting that BHV-4 and HVS are evolutionarily more closely related to each other than either are to EBV. BHV-4 DNA sequences were generally deficient in CpG dinucleotide. This CpG deficiency is characteristic of gammaherpesvirus genomes and suggests that the BHV-4 latent genome is extensively methylated. Despite several biological features similar to those of beta herpesviruses, BHV-4 displays the molecular characteristics of the representative members of the gammaherpesvirinae subfamily.
AB - The overall arrangement of genes in the unique central part of the bovine herpesvirus type 4 (BHV-4) genome has been deduced by analysis of short DNA sequences. Twenty-three genes conserved in at least one of the completely sequenced herpesviruses have been identified and localized. All of these genes encoded amino acid sequences with higher similarity to proteins of the gammaherpesviruses Epstein-Barr virus (EBV) and herpesvirus saimiri (HVS) than to the homologous products of the alphaherpesviruses varicella-zoster virus and herpes simplex virus type 1 or the betaherpesvirus human cytomegalovirus. The genome organization of BHV-4 had also an overall collinearity with that of the gammaherpesviruses EBV and HVS. Furthermore, the BHV-4 genes content and arrangement were more similar to those of HVS than to those of EBV, suggesting that BHV-4 and HVS are evolutionarily more closely related to each other than either are to EBV. BHV-4 DNA sequences were generally deficient in CpG dinucleotide. This CpG deficiency is characteristic of gammaherpesvirus genomes and suggests that the BHV-4 latent genome is extensively methylated. Despite several biological features similar to those of beta herpesviruses, BHV-4 displays the molecular characteristics of the representative members of the gammaherpesvirinae subfamily.
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U2 - 10.1016/0042-6822(92)90903-3
DO - 10.1016/0042-6822(92)90903-3
M3 - Article
C2 - 1325698
AN - SCOPUS:0026757390
SN - 0042-6822
VL - 190
SP - 654
EP - 665
JO - Virology
JF - Virology
IS - 2
ER -