Genetic mosaic analysis of stem cell lineages in the Drosophila ovary

Kaitlin M. Laws; Daniela Drummond-Barbosa

doi:10.1007/978-1-4939-2851-4_4

Genetic mosaic analysis of stem cell lineages in the Drosophila ovary

Kaitlin M. Laws, Daniela Drummond-Barbosa

Bloomberg School of Public Health

Research output: Chapter in Book/Report/Conference proceeding › Chapter

10 Scopus citations

Abstract

Genetic mosaic analyses represent an invaluable approach for the study of stem cell lineages in the Drosophila ovary. The generation of readily identifiable, homozygous mutant cells in the context of wild- type ovarian tissues within intact organisms allows the pinpointing of cellular requirements for gene function, which is particularly important for understanding the physiological control of stem cells and their progeny. Here, we provide a step-by-step guide to the generation and analysis of genetically mosaic ovaries using flippase (FLP)/ FLP recognition target ( FRT )-mediated recombination in adult Drosophila melanogaster, with a focus on the processes of oogenesis that are controlled by diet-dependent factors.

Original language	English (US)
Title of host publication	Drosophila Oogenesis
Subtitle of host publication	Methods and Protocols
Publisher	Springer New York
Pages	57-72
Number of pages	16
ISBN (Electronic)	9781493928514
ISBN (Print)	9781493928507
DOIs	https://doi.org/10.1007/978-1-4939-2851-4_4
State	Published - Aug 31 2015

Keywords

Clonal analysis
Drosophila
FLP/FRT
Follicle cell
Follicle stem cell
Genetic mosaics
Germline stem cell
Oogenesis

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Agricultural and Biological Sciences
General Medicine

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10.1007/978-1-4939-2851-4_4

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abstract = "Genetic mosaic analyses represent an invaluable approach for the study of stem cell lineages in the Drosophila ovary. The generation of readily identifiable, homozygous mutant cells in the context of wild- type ovarian tissues within intact organisms allows the pinpointing of cellular requirements for gene function, which is particularly important for understanding the physiological control of stem cells and their progeny. Here, we provide a step-by-step guide to the generation and analysis of genetically mosaic ovaries using flippase (FLP)/ FLP recognition target ( FRT )-mediated recombination in adult Drosophila melanogaster, with a focus on the processes of oogenesis that are controlled by diet-dependent factors.",

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Genetic mosaic analysis of stem cell lineages in the Drosophila ovary

Abstract

Keywords

ASJC Scopus subject areas

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