Abstract
Genetic mosaic analyses represent an invaluable approach for the study of stem cell lineages in the Drosophila ovary. The generation of readily identifiable, homozygous mutant cells in the context of wild- type ovarian tissues within intact organisms allows the pinpointing of cellular requirements for gene function, which is particularly important for understanding the physiological control of stem cells and their progeny. Here, we provide a step-by-step guide to the generation and analysis of genetically mosaic ovaries using flippase (FLP)/ FLP recognition target ( FRT )-mediated recombination in adult Drosophila melanogaster, with a focus on the processes of oogenesis that are controlled by diet-dependent factors.
| Original language | English (US) |
|---|---|
| Title of host publication | Drosophila Oogenesis |
| Subtitle of host publication | Methods and Protocols |
| Publisher | Springer New York |
| Pages | 57-72 |
| Number of pages | 16 |
| ISBN (Electronic) | 9781493928514 |
| ISBN (Print) | 9781493928507 |
| DOIs | |
| State | Published - Aug 31 2015 |
Keywords
- Clonal analysis
- Drosophila
- FLP/FRT
- Follicle cell
- Follicle stem cell
- Genetic mosaics
- Germline stem cell
- Oogenesis
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Agricultural and Biological Sciences(all)
- Medicine(all)