Genetic construction, expression, and melanoma-selective cytotoxicity of a diphtheria toxin-related α-melanocyte-stimulating hormone fusion protein

J. R. Murphy; W. Bishai; M. Borowski; A. Miyanohara; J. Boyd; S. Nagle

doi:10.1073/pnas.83.21.8258

Genetic construction, expression, and melanoma-selective cytotoxicity of a diphtheria toxin-related α-melanocyte-stimulating hormone fusion protein

J. R. Murphy, W. Bishai, M. Borowski, A. Miyanohara, J. Boyd, S. Nagle

Research output: Contribution to journal › Article › peer-review

106 Scopus citations

Abstract

The structural gene for diphtheria toxin, tox, has been modified at its Sph I site by the introduction of an oligonucleotide linker encoding a unique Pst I restriction endonuclease site and a synthetic oligonucleotide encoding α-melanocyte-stimulating hormone (α-MSH). The resulting fusion gene directs the expression of a diphtheria toxin-related α-MSH hybrid protein in which the diphtheria toxin receptor-binding domain has been replaced with α-MSH sequences. The chimeric toxin has been partially purified from periplasmic extracts of recombinant Escherichia coli K-12 and has been found to be selectively toxic for α-MSH receptor-positive human malignant melanoma NEL-M1 cells in vitro.

Original language	English (US)
Pages (from-to)	8258-8262
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	83
Issue number	21
DOIs	https://doi.org/10.1073/pnas.83.21.8258
State	Published - 1986
Externally published	Yes

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Murphy, J. R., Bishai, W., Borowski, M., Miyanohara, A., Boyd, J., & Nagle, S. (1986). Genetic construction, expression, and melanoma-selective cytotoxicity of a diphtheria toxin-related α-melanocyte-stimulating hormone fusion protein. Proceedings of the National Academy of Sciences of the United States of America, 83(21), 8258-8262. https://doi.org/10.1073/pnas.83.21.8258

Genetic construction, expression, and melanoma-selective cytotoxicity of a diphtheria toxin-related α-melanocyte-stimulating hormone fusion protein. / Murphy, J. R.; Bishai, W.; Borowski, M. et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, No. 21, 1986, p. 8258-8262.

Research output: Contribution to journal › Article › peer-review

Murphy, JR, Bishai, W, Borowski, M, Miyanohara, A, Boyd, J & Nagle, S 1986, 'Genetic construction, expression, and melanoma-selective cytotoxicity of a diphtheria toxin-related α-melanocyte-stimulating hormone fusion protein', Proceedings of the National Academy of Sciences of the United States of America, vol. 83, no. 21, pp. 8258-8262. https://doi.org/10.1073/pnas.83.21.8258

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Genetic construction, expression, and melanoma-selective cytotoxicity of a diphtheria toxin-related α-melanocyte-stimulating hormone fusion protein

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