Genetic analysis of digestive physiology using fluorescent phospholipid reporters

Steven A. Farber; Michael Pack; Shiu Ying Ho; Iain D. Johnson; Daniel S. Wagner; Roland Dosch; Mary C. Mullins; H. Stewart Hendrickson; Elizabeth K. Hendrickson; Marnie E. Halpern

doi:10.1126/science.1060418

Genetic analysis of digestive physiology using fluorescent phospholipid reporters

Steven A. Farber, Michael Pack, Shiu Ying Ho, Iain D. Johnson, Daniel S. Wagner, Roland Dosch, Mary C. Mullins, H. Stewart Hendrickson, Elizabeth K. Hendrickson, Marnie E. Halpern

Research output: Contribution to journal › Article › peer-review

263 Scopus citations

Abstract

Zebrafish are a valuable model for mammalian lipid metabolism; larvae process lipids similarly through the intestine and hepatobiliary system and respond to drugs that block cholesterol synthesis in humans. After ingestion of fluorescently quenched phospholipids, endogenous lipase activity and rapid transport of cleavage products results in intense gall bladder fluorescence. Genetic screening identifies zebrafish mutants, such as fat free, that show normal digestive organ morphology but severely reduced phospholipid and cholesterol processing. Thus, fluorescent lipids provide a sensitive readout of lipid metabolism and are a powerful tool for identifying genes that mediate vertebrate digestive physiology.

Original language	English (US)
Pages (from-to)	1385-1388
Number of pages	4
Journal	Science
Volume	292
Issue number	5520
DOIs	https://doi.org/10.1126/science.1060418
State	Published - May 18 2001
Externally published	Yes

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title = "Genetic analysis of digestive physiology using fluorescent phospholipid reporters",

abstract = "Zebrafish are a valuable model for mammalian lipid metabolism; larvae process lipids similarly through the intestine and hepatobiliary system and respond to drugs that block cholesterol synthesis in humans. After ingestion of fluorescently quenched phospholipids, endogenous lipase activity and rapid transport of cleavage products results in intense gall bladder fluorescence. Genetic screening identifies zebrafish mutants, such as fat free, that show normal digestive organ morphology but severely reduced phospholipid and cholesterol processing. Thus, fluorescent lipids provide a sensitive readout of lipid metabolism and are a powerful tool for identifying genes that mediate vertebrate digestive physiology.",

author = "Farber, {Steven A.} and Michael Pack and Ho, {Shiu Ying} and Johnson, {Iain D.} and Wagner, {Daniel S.} and Roland Dosch and Mullins, {Mary C.} and Hendrickson, {H. Stewart} and Hendrickson, {Elizabeth K.} and Halpern, {Marnie E.}",

year = "2001",

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doi = "10.1126/science.1060418",

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volume = "292",

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T1 - Genetic analysis of digestive physiology using fluorescent phospholipid reporters

AU - Farber, Steven A.

AU - Pack, Michael

AU - Ho, Shiu Ying

AU - Johnson, Iain D.

AU - Wagner, Daniel S.

AU - Dosch, Roland

AU - Mullins, Mary C.

AU - Hendrickson, H. Stewart

AU - Hendrickson, Elizabeth K.

AU - Halpern, Marnie E.

PY - 2001/5/18

Y1 - 2001/5/18

N2 - Zebrafish are a valuable model for mammalian lipid metabolism; larvae process lipids similarly through the intestine and hepatobiliary system and respond to drugs that block cholesterol synthesis in humans. After ingestion of fluorescently quenched phospholipids, endogenous lipase activity and rapid transport of cleavage products results in intense gall bladder fluorescence. Genetic screening identifies zebrafish mutants, such as fat free, that show normal digestive organ morphology but severely reduced phospholipid and cholesterol processing. Thus, fluorescent lipids provide a sensitive readout of lipid metabolism and are a powerful tool for identifying genes that mediate vertebrate digestive physiology.

AB - Zebrafish are a valuable model for mammalian lipid metabolism; larvae process lipids similarly through the intestine and hepatobiliary system and respond to drugs that block cholesterol synthesis in humans. After ingestion of fluorescently quenched phospholipids, endogenous lipase activity and rapid transport of cleavage products results in intense gall bladder fluorescence. Genetic screening identifies zebrafish mutants, such as fat free, that show normal digestive organ morphology but severely reduced phospholipid and cholesterol processing. Thus, fluorescent lipids provide a sensitive readout of lipid metabolism and are a powerful tool for identifying genes that mediate vertebrate digestive physiology.

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Genetic analysis of digestive physiology using fluorescent phospholipid reporters

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