@inbook{73e6ec3c51fa4428b4a9e8b5c5523785,
title = "Generation of Pericytic-Vascular Progenitors from Tankyrase/PARP-Inhibitor-Regulated Na{\"i}ve (TIRN) Human Pluripotent Stem Cells",
abstract = "Tankyrase/PARP inhibitor-regulated na{\"i}ve human pluripotent stem cells (TIRN-hPSC) represent a new class of human stem cells for regenerative medicine that can differentiate into multi-lineage progenitors with improved in vivo functionality. Chemical reversion of conventional, primed hPSC to a TIRN-hPSC state alleviates dysfunctional epigenetic donor cell memory, lineage-primed gene expression, and potentially disease-associated aberrations in their differentiated progeny. Here, we provide methods for the reversion of normal or diseased patient-specific primed hPSC to TIRN-hPSC and describe their subsequent differentiation into embryonic-like pericytic-endothelial “na{\"i}ve” vascular progenitors (N-VP). N-VP possess improved vascular functionality, high epigenetic plasticity, maintain greater genomic stability, and are more efficient in migrating to and re-vascularizing ischemic tissues than those generated from primed isogenic hPSC. We also describe detailed methods for the ocular transplantation and quantitation of vascular engraftment of N-VP into the ischemia-damaged neural retina of a humanized mouse model of ischemic retinopathy. The application of TIRN-hPSC-derived N-VP will advance vascular cell therapies of ischemic retinopathy, myocardial infarction, and cerebral vascular stroke.",
keywords = "Differentiation, Human pluripotent stem cell, Ischemic retinopathy, Na{\"i}ve pluripotency, PARP, Pericyte, Tankyrase inhibition, Vascular progenitors, Vascular regeneration",
author = "Ludovic Zimmerlin and Park, {Tea Soon} and Imran Bhutto and Gerard Lutty and Zambidis, {Elias T.}",
note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-1908-7_10",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "133--156",
booktitle = "Methods in Molecular Biology",
}