Generation and molecular cytogenetic characterization of a radiation-reduction hybrid panel for human chromosome 6

G. J. Pappas; E. Thompson; A. Burgess; A. Greenwood; J. M. Trent

doi:10.1159/000133963

Generation and molecular cytogenetic characterization of a radiation-reduction hybrid panel for human chromosome 6

G. J. Pappas, E. Thompson, A. Burgess, A. Greenwood, J. M. Trent

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

A neo^R marked chromosome-6 containing hybrid (D113 JA) was used to generate a panel of 15 radiation-reduced hybrid cell lines. The panel was constructed by irradiating microcells isolated from D113JA at 800 or 8000 rads, providing different levels of chromosome 6 retention. These hybrids have been systematically analyzed using interspersed repetitive elements, previously assigned markers for chromosome 6, and fluorescent in situ hybridization (FISH). As expected, G418 selection has favored the retention of fragments near the insertion site of the neo^R gene (6q16). The panel as constituted provides an important resource for regional assignment of molecular markers, especially to regions on 6q.

Original language	English (US)
Pages (from-to)	201-206
Number of pages	6
Journal	Cytogenetics and cell genetics
Volume	69
Issue number	3-4
DOIs	https://doi.org/10.1159/000133963
State	Published - 1995
Externally published	Yes

ASJC Scopus subject areas

Genetics
Cell Biology

Access to Document

10.1159/000133963

Cite this

@article{b7d6603e82f94268847e7671fcace894,

title = "Generation and molecular cytogenetic characterization of a radiation-reduction hybrid panel for human chromosome 6",

abstract = "A neoR marked chromosome-6 containing hybrid (D113 JA) was used to generate a panel of 15 radiation-reduced hybrid cell lines. The panel was constructed by irradiating microcells isolated from D113JA at 800 or 8000 rads, providing different levels of chromosome 6 retention. These hybrids have been systematically analyzed using interspersed repetitive elements, previously assigned markers for chromosome 6, and fluorescent in situ hybridization (FISH). As expected, G418 selection has favored the retention of fragments near the insertion site of the neoR gene (6q16). The panel as constituted provides an important resource for regional assignment of molecular markers, especially to regions on 6q.",

author = "Pappas, {G. J.} and E. Thompson and A. Burgess and A. Greenwood and Trent, {J. M.}",

year = "1995",

doi = "10.1159/000133963",

language = "English (US)",

volume = "69",

pages = "201--206",

journal = "Cytogenetics and cell genetics",

issn = "0301-0171",

publisher = "S. Karger AG",

number = "3-4",

}

TY - JOUR

T1 - Generation and molecular cytogenetic characterization of a radiation-reduction hybrid panel for human chromosome 6

AU - Pappas, G. J.

AU - Thompson, E.

AU - Burgess, A.

AU - Greenwood, A.

AU - Trent, J. M.

PY - 1995

Y1 - 1995

N2 - A neoR marked chromosome-6 containing hybrid (D113 JA) was used to generate a panel of 15 radiation-reduced hybrid cell lines. The panel was constructed by irradiating microcells isolated from D113JA at 800 or 8000 rads, providing different levels of chromosome 6 retention. These hybrids have been systematically analyzed using interspersed repetitive elements, previously assigned markers for chromosome 6, and fluorescent in situ hybridization (FISH). As expected, G418 selection has favored the retention of fragments near the insertion site of the neoR gene (6q16). The panel as constituted provides an important resource for regional assignment of molecular markers, especially to regions on 6q.

AB - A neoR marked chromosome-6 containing hybrid (D113 JA) was used to generate a panel of 15 radiation-reduced hybrid cell lines. The panel was constructed by irradiating microcells isolated from D113JA at 800 or 8000 rads, providing different levels of chromosome 6 retention. These hybrids have been systematically analyzed using interspersed repetitive elements, previously assigned markers for chromosome 6, and fluorescent in situ hybridization (FISH). As expected, G418 selection has favored the retention of fragments near the insertion site of the neoR gene (6q16). The panel as constituted provides an important resource for regional assignment of molecular markers, especially to regions on 6q.

UR - http://www.scopus.com/inward/record.url?scp=0028967031&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028967031&partnerID=8YFLogxK

U2 - 10.1159/000133963

DO - 10.1159/000133963

M3 - Article

C2 - 7698012

AN - SCOPUS:0028967031

SN - 0301-0171

VL - 69

SP - 201

EP - 206

JO - Cytogenetics and cell genetics

JF - Cytogenetics and cell genetics

IS - 3-4

ER -

Generation and molecular cytogenetic characterization of a radiation-reduction hybrid panel for human chromosome 6

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this