TY - JOUR
T1 - Generation and characterization of a cell line from an intraductal tubulopapillary neoplasm of the pancreas
AU - Felsenstein, Matthäus
AU - Trujillo, Maria A.
AU - Huang, Bo
AU - Nanda, Neha
AU - Jiang, Zhengdong
AU - Jeong, Yea Ji
AU - Pflüger, Michael
AU - Goggins, Michael G.
AU - Hruban, Ralph H.
AU - Thompson, Elizabeth D.
AU - Heaphy, Christopher M.
AU - Roberts, Nicholas J.
AU - Wood, Laura D.
N1 - Funding Information:
Acknowledgements We thank Dr James R. Eshleman for helpful discussions. The authors acknowledge the following sources of support: Rolfe Pancreatic Cancer Foundation; Susan Wojcicki and Denis Troper; NIH/NCI P50 CA62924; NIH/NIDDK K08 DK107781; NIH/ NCI R00 CA190889; Sol Goldman Pancreatic Cancer Research Center; DFG-German Research Foundation; BIH-Charité Junior Clinician Scientist Program; Buffone Family Gastrointestinal Cancer Research Fund; Kaya Tuncer Career Development Award in Gastrointestinal Cancer Prevention; AGA-Bernard Lee Schwartz Foundation Research Scholar Award in Pancreatic Cancer; Sidney Kimmel Foundation for Cancer Research Kimmel Scholar Award; AACR-Incyte Corporation Career Development Award for Pancreatic Cancer Research; American Cancer Society Research Scholar Grant; Emerson Collective Cancer Research Fund; Joseph C Monastra Foundation; The Gerald O Mann Charitable Foundation (Harriet and Allan Wulfstat, Trustees); Art Creates Cures Foundation.
Publisher Copyright:
© 2020, The Author(s), under exclusive licence to United States and Canadian Academy of Pathology.
PY - 2020/7/1
Y1 - 2020/7/1
N2 - Intraductal tubulopapillary neoplasm (ITPN) is a distinct precancerous lesion in the pancreas with unique clinical and molecular features. Although in vitro studies in two-dimensional culture have led to numerous important insights in pancreatic cancer, such models are currently lacking for precancerous lesions. In this study, we report the generation and characterization of a cell line from a human pancreatic ITPN. Neoplastic cells were initially cultured in a three-dimensional organoid system, followed by transfer to two-dimensional culture. RNA sequencing revealed a gene expression profile consistent with pancreatic ductal origin, and whole genome sequencing identified many somatic mutations (including in genes involved in DNA repair and Wnt signaling) and structural rearrangements. In vitro characterization of the tumorigenic potential demonstrated a phenotype between that of normal pancreatic ductal cells and cancer cell lines. This cell line represents a valuable resource for interrogation of unique ITPN biology, as well as precancerous pancreatic lesions more generally.
AB - Intraductal tubulopapillary neoplasm (ITPN) is a distinct precancerous lesion in the pancreas with unique clinical and molecular features. Although in vitro studies in two-dimensional culture have led to numerous important insights in pancreatic cancer, such models are currently lacking for precancerous lesions. In this study, we report the generation and characterization of a cell line from a human pancreatic ITPN. Neoplastic cells were initially cultured in a three-dimensional organoid system, followed by transfer to two-dimensional culture. RNA sequencing revealed a gene expression profile consistent with pancreatic ductal origin, and whole genome sequencing identified many somatic mutations (including in genes involved in DNA repair and Wnt signaling) and structural rearrangements. In vitro characterization of the tumorigenic potential demonstrated a phenotype between that of normal pancreatic ductal cells and cancer cell lines. This cell line represents a valuable resource for interrogation of unique ITPN biology, as well as precancerous pancreatic lesions more generally.
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U2 - 10.1038/s41374-020-0372-0
DO - 10.1038/s41374-020-0372-0
M3 - Article
C2 - 32005909
AN - SCOPUS:85078920713
SN - 0023-6837
VL - 100
SP - 1003
EP - 1013
JO - Laboratory Investigation
JF - Laboratory Investigation
IS - 7
ER -