@inbook{d793d1a906264da4b3ff55da8a419acc,
title = "Generating mammalian stable cell lines by electroporation",
abstract = "Expression of functional, recombinant mammalian proteins often requires expression in mammalian cells (see Single Cell Cloning of a Stable Mammalian Cell Line). If the expressed protein needs to be made frequently, it can be best to generate a stable cell line instead of performing repeated transient transfections into mammalian cells. Here, we describe a method to generate stable cell lines via electroporation followed by selection steps. This protocol will be limited to the CHO dhfr- Urlaub et al. (1983) and LEC1 cell lines, which in our experience perform the best with this method.",
author = "{A. Longo}, Patti and Kavran, {Jennifer M.} and Kim, {Min Sung} and Leahy, {Daniel J}",
year = "2013",
doi = "10.1016/B978-0-12-418687-3.00017-3",
language = "English (US)",
isbn = "9780124186873",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "209--226",
booktitle = "Laboratory Methods in Enzymology",
}