TY - JOUR
T1 - Gene polymorphisms of tumor necrosis factor alpha-308 and interleukin-10-1082 among asthmatic Egyptian children
AU - Zedan, Magdy
AU - Settin, Ahmed
AU - Farag, Mohammad K.
AU - El-Bayoumi, Mohammed
AU - El Regal, Mohammed Ezz
AU - El Baz, Rizk
AU - Osman, Engy
PY - 2008/5
Y1 - 2008/5
N2 - Tumor necrosis factor (TNF) alpha-308 and interleukin (IL)-10-1082 have potent inflammatory responses in the process of airway inflammation in asthma. The purpose of this study was to check for association of polymorphisms related to cytokine genes with susceptibility and severity of bronchial asthma in Egyptian children. Blood samples of 69 asthmatic children receiving treatment and follow-up at the Allergy and Respiratory Medicine Unit, Mansoura University Children Hospital, Mansoura, Egypt, were subjected to DNA extraction and amplification using polymerase chain reaction with sequence-specific primers for detection of single nucleotide polymorphisms in the promoter regions of cytokine genes TNF-alpha-308(G→A), IL-10-1082(G→A). Compared with normal controls, Egyptian asthmatic children showed a significant higher frequency of IL-10 -1082 G/G homozygosity genotype (p <0.001; odds ratio [OR] = 7) with lower frequency of G/A heterozygosity genotype among cases. This finding also was detected in cases with persistent asthma and eczema. These cases showed significant lower frequency of TNF-alpha-308 G/A heterozygosity (p <0.05; OR = 0.44). Also, male cases, cases with positive family history, and those patients with persistent types of asthma showed a higher frequency of TNF-alpha-308 G/G homozygosity. H-10-1082(G→A) G/G and TNF-alpha-308(G→A) G/G may be a contributing factor in susceptibility as well as severity of asthma among Egyptian children. Separate studies should be specified relating these cytokine genotypes to response to various modalities in asthma therapy. This study reports that IL-10- 1082(G→A) G/G and TNF-alpha-308(G→A) G/G genotypes may be contributing factors in susceptibility as well as in severity of asthma among Egyptian children. Separate studies may be specified relating these cytokine genotypes to response to various modalities in asthma therapy.
AB - Tumor necrosis factor (TNF) alpha-308 and interleukin (IL)-10-1082 have potent inflammatory responses in the process of airway inflammation in asthma. The purpose of this study was to check for association of polymorphisms related to cytokine genes with susceptibility and severity of bronchial asthma in Egyptian children. Blood samples of 69 asthmatic children receiving treatment and follow-up at the Allergy and Respiratory Medicine Unit, Mansoura University Children Hospital, Mansoura, Egypt, were subjected to DNA extraction and amplification using polymerase chain reaction with sequence-specific primers for detection of single nucleotide polymorphisms in the promoter regions of cytokine genes TNF-alpha-308(G→A), IL-10-1082(G→A). Compared with normal controls, Egyptian asthmatic children showed a significant higher frequency of IL-10 -1082 G/G homozygosity genotype (p <0.001; odds ratio [OR] = 7) with lower frequency of G/A heterozygosity genotype among cases. This finding also was detected in cases with persistent asthma and eczema. These cases showed significant lower frequency of TNF-alpha-308 G/A heterozygosity (p <0.05; OR = 0.44). Also, male cases, cases with positive family history, and those patients with persistent types of asthma showed a higher frequency of TNF-alpha-308 G/G homozygosity. H-10-1082(G→A) G/G and TNF-alpha-308(G→A) G/G may be a contributing factor in susceptibility as well as severity of asthma among Egyptian children. Separate studies should be specified relating these cytokine genotypes to response to various modalities in asthma therapy. This study reports that IL-10- 1082(G→A) G/G and TNF-alpha-308(G→A) G/G genotypes may be contributing factors in susceptibility as well as in severity of asthma among Egyptian children. Separate studies may be specified relating these cytokine genotypes to response to various modalities in asthma therapy.
KW - Asthma
KW - Children
KW - Egypt
KW - Gene polymorphisms
KW - IL 10
KW - TNF-alpha
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U2 - 10.2500/aap.2008.29.3123
DO - 10.2500/aap.2008.29.3123
M3 - Article
C2 - 18534084
AN - SCOPUS:46649104250
SN - 1088-5412
VL - 29
SP - 268
EP - 273
JO - Allergy and Asthma Proceedings
JF - Allergy and Asthma Proceedings
IS - 3
ER -