Gene polymorphisms of IL-6-174 G/C and IL-1Ra VNTR in asthmatic children

Ahmad Settin; Magdy Zedan; Mohamed Farag; Mohammed Ezz El Regal; Engy Osman

doi:10.1007/s12098-008-0161-z

Gene polymorphisms of IL-6-174 G/C and IL-1Ra VNTR in asthmatic children

Ahmad Settin, Magdy Zedan, Mohamed Farag, Mohammed Ezz El Regal, Engy Osman

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Objective. To check for the association of genetic polymorphisms of IL-6-^-174G/C and IL-1RaVNTR with the susceptibility and severity of asthma in Egyptian children. Methods. Subjects included 69 asthmatic children and 98 healthy unrelated controls from the Nile Delta of Egypt. Cases consisted of 20 males and 49 females with an age mean± SD is 7.5 ± 2.1 ranging between 2-13 years. DNA amplification using PCR with sequence-specific primers was done for detection of promotor single nucleotide polymorphism of IL-6 gene as well as intron 2 VNTR of IL-1Ra gene. Frequency of case-genotypes or alleles were compared to controls using Fisher exact test and Odds ratio. Results. Cases showed significant higher frequency of the genotypes: IL-6-174 GG (P-174 GC and IL-1Ra A1A2 genotypes (P

Original language	English (US)
Pages (from-to)	1019-1023
Number of pages	5
Journal	Indian Journal of Pediatrics
Volume	75
Issue number	10
DOIs	https://doi.org/10.1007/s12098-008-0161-z
State	Published - Oct 2008
Externally published	Yes

Keywords

Childhood asthma
Egypt
Interleukins
Polymorphism

ASJC Scopus subject areas

Pediatrics, Perinatology, and Child Health

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10.1007/s12098-008-0161-z

Cite this

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title = "Gene polymorphisms of IL-6-174 G/C and IL-1Ra VNTR in asthmatic children",

abstract = "Objective. To check for the association of genetic polymorphisms of IL-6--174G/C and IL-1RaVNTR with the susceptibility and severity of asthma in Egyptian children. Methods. Subjects included 69 asthmatic children and 98 healthy unrelated controls from the Nile Delta of Egypt. Cases consisted of 20 males and 49 females with an age mean± SD is 7.5 ± 2.1 ranging between 2-13 years. DNA amplification using PCR with sequence-specific primers was done for detection of promotor single nucleotide polymorphism of IL-6 gene as well as intron 2 VNTR of IL-1Ra gene. Frequency of case-genotypes or alleles were compared to controls using Fisher exact test and Odds ratio. Results. Cases showed significant higher frequency of the genotypes: IL-6-174 GG (P-174 GC and IL-1Ra A1A2 genotypes (P",

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author = "Ahmad Settin and Magdy Zedan and Mohamed Farag and {Ezz El Regal}, Mohammed and Engy Osman",

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doi = "10.1007/s12098-008-0161-z",

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AU - Settin, Ahmad

AU - Zedan, Magdy

AU - Farag, Mohamed

AU - Ezz El Regal, Mohammed

AU - Osman, Engy

PY - 2008/10

Y1 - 2008/10

N2 - Objective. To check for the association of genetic polymorphisms of IL-6--174G/C and IL-1RaVNTR with the susceptibility and severity of asthma in Egyptian children. Methods. Subjects included 69 asthmatic children and 98 healthy unrelated controls from the Nile Delta of Egypt. Cases consisted of 20 males and 49 females with an age mean± SD is 7.5 ± 2.1 ranging between 2-13 years. DNA amplification using PCR with sequence-specific primers was done for detection of promotor single nucleotide polymorphism of IL-6 gene as well as intron 2 VNTR of IL-1Ra gene. Frequency of case-genotypes or alleles were compared to controls using Fisher exact test and Odds ratio. Results. Cases showed significant higher frequency of the genotypes: IL-6-174 GG (P-174 GC and IL-1Ra A1A2 genotypes (P

AB - Objective. To check for the association of genetic polymorphisms of IL-6--174G/C and IL-1RaVNTR with the susceptibility and severity of asthma in Egyptian children. Methods. Subjects included 69 asthmatic children and 98 healthy unrelated controls from the Nile Delta of Egypt. Cases consisted of 20 males and 49 females with an age mean± SD is 7.5 ± 2.1 ranging between 2-13 years. DNA amplification using PCR with sequence-specific primers was done for detection of promotor single nucleotide polymorphism of IL-6 gene as well as intron 2 VNTR of IL-1Ra gene. Frequency of case-genotypes or alleles were compared to controls using Fisher exact test and Odds ratio. Results. Cases showed significant higher frequency of the genotypes: IL-6-174 GG (P-174 GC and IL-1Ra A1A2 genotypes (P

KW - Childhood asthma

KW - Egypt

KW - Interleukins

KW - Polymorphism

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Gene polymorphisms of IL-6-174 G/C and IL-1Ra VNTR in asthmatic children

Abstract

Keywords

ASJC Scopus subject areas

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