Gel Scaffolds of BMP-2-Binding Peptide Amphiphile Nanofibers for Spinal Arthrodesis

Sungsoo S. Lee, Erin L. Hsu, Marco Mendoza, Jason Ghodasra, Michael S. Nickoli, Amruta Ashtekar, Mahesh Polavarapu, Jacob Babu, Rehan M. Riaz, Joseph D. Nicolas, David Nelson, Sohaib Z. Hashmi, Stuart R. Kaltz, Jeffrey S. Earhart, Bradley R. Merk, Jeff S. Mckee, Shawn F. Bairstow, Ramille N. Shah, Wellington K. Hsu, Samuel I. Stupp

Research output: Contribution to journalArticlepeer-review

112 Scopus citations


Peptide amphiphile (PA) nanofibers formed by self-assembly can be customized for specific applications in regenerative medicine through the use of molecules that display bioactive signals on their surfaces. Here, the use of PA nanofibers with binding affinity for the bone promoting growth factor BMP-2 to create a gel scaffold for osteogenesis is reported. With the objective of reducing the amount of BMP-2 used clinically for successful arthrodesis in the spine, amounts of growth factor incorporated in the scaffolds that are 10 to 100 times lower than that those used clinically in collagen scaffolds are used. The efficacy of the bioactive PA system to promote BMP-2-induced osteogenesis in vivo is investigated in a rat posterolateral lumbar intertransverse spinal fusion model. PA nanofiber gels displaying BMP-2-binding segments exhibit superior spinal fusion rates relative to controls, effectively decreasing the required therapeutic dose of BMP-2 by 10-fold. Interestingly, a 42% fusion rate is observed for gels containing the bioactive nanofibers without the use of exogenous BMP-2, suggesting the ability of the nanofiber to recruit endogenous growth factor. Results obtained here demonstrate that bioactive biomaterials with capacity to bind specific growth factors by design are great targets for regenerative medicine. Supramolecular nanofibers presenting BMP-2-binding epitopes on the surface exhibit superior spinal fusion rates in rats, effectively decreasing the therapeutic dose of BMP-2 by 10-fold. Importantly, the bioactive nanofibers elicit 42% fusion rate without the addition of exogenous BMP-2.

Original languageEnglish (US)
Pages (from-to)131-141
Number of pages11
JournalAdvanced Healthcare Materials
Issue number1
StatePublished - Jan 1 2015
Externally publishedYes


  • BMP-2 (bone morphogenetic protein-2)
  • Bone regeneration
  • Peptide amphiphile
  • Regenerative medicine
  • Spinal fusion

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Pharmaceutical Science


Dive into the research topics of 'Gel Scaffolds of BMP-2-Binding Peptide Amphiphile Nanofibers for Spinal Arthrodesis'. Together they form a unique fingerprint.

Cite this