Gas-Phase Scrambling of Disulfide Bonds during Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Analysis

Liang Zhao; Ruben T. Almaraz; Fan Xiang; Jerry L. Hedrick; Andreas H. Franz

doi:10.1016/j.jasms.2009.04.021

Gas-Phase Scrambling of Disulfide Bonds during Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Analysis

Liang Zhao, Ruben T. Almaraz, Fan Xiang, Jerry L. Hedrick, Andreas H. Franz

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Evidence for photo-induced radical disulfide bond scrambling in the gas phase during matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is described. The phenomenon was observed during the analysis of tryptic peptides from insulin and was confirmed in the determination of disulfide bonds in the rhamnose-binding lectin SEL24K from the Chinook salmon Oncorhynchus tshawytscha. A possible mechanism for this surprising scrambling is proposed. Despite this finding, the disulfide bond pattern in SEL24K was assigned unambiguously by a multi-enzyme digestion strategy in combination with MALDI mass spectrometry. The pattern was found to be symmetrical in the tandem repeat sequence of SEL24K. To the best of our knowledge, this is the first report of disulfide bond scrambling in the gas phase during MALDI-MS analysis. This observation has important ramifications for unambiguous assignment of disulfide bonds.

Original language	English (US)
Pages (from-to)	1603-1616
Number of pages	14
Journal	Journal of the American Society for Mass Spectrometry
Volume	20
Issue number	9
DOIs	https://doi.org/10.1016/j.jasms.2009.04.021
State	Published - Sep 2009
Externally published	Yes

ASJC Scopus subject areas

Structural Biology
Spectroscopy

Access to Document

10.1016/j.jasms.2009.04.021

Cite this

@article{87ca3437105e430784f2462d34d020e4,

title = "Gas-Phase Scrambling of Disulfide Bonds during Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Analysis",

abstract = "Evidence for photo-induced radical disulfide bond scrambling in the gas phase during matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is described. The phenomenon was observed during the analysis of tryptic peptides from insulin and was confirmed in the determination of disulfide bonds in the rhamnose-binding lectin SEL24K from the Chinook salmon Oncorhynchus tshawytscha. A possible mechanism for this surprising scrambling is proposed. Despite this finding, the disulfide bond pattern in SEL24K was assigned unambiguously by a multi-enzyme digestion strategy in combination with MALDI mass spectrometry. The pattern was found to be symmetrical in the tandem repeat sequence of SEL24K. To the best of our knowledge, this is the first report of disulfide bond scrambling in the gas phase during MALDI-MS analysis. This observation has important ramifications for unambiguous assignment of disulfide bonds.",

author = "Liang Zhao and Almaraz, {Ruben T.} and Fan Xiang and Hedrick, {Jerry L.} and Franz, {Andreas H.}",

note = "Funding Information: The authors thank the Department of Chemistry for support and Dr. Kiran Morishetti for help with the electrospray measurements. They are grateful to Thermo-Fisher for access to the TSQ mass spectrometer. R.T.A. was supported by a University of California Dissertation Year Fellowship. ",

year = "2009",

month = sep,

doi = "10.1016/j.jasms.2009.04.021",

language = "English (US)",

volume = "20",

pages = "1603--1616",

journal = "Journal of the American Society for Mass Spectrometry",

issn = "1044-0305",

publisher = "Springer New York",

number = "9",

}

TY - JOUR

T1 - Gas-Phase Scrambling of Disulfide Bonds during Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Analysis

AU - Zhao, Liang

AU - Almaraz, Ruben T.

AU - Xiang, Fan

AU - Hedrick, Jerry L.

AU - Franz, Andreas H.

N1 - Funding Information: The authors thank the Department of Chemistry for support and Dr. Kiran Morishetti for help with the electrospray measurements. They are grateful to Thermo-Fisher for access to the TSQ mass spectrometer. R.T.A. was supported by a University of California Dissertation Year Fellowship.

PY - 2009/9

Y1 - 2009/9

N2 - Evidence for photo-induced radical disulfide bond scrambling in the gas phase during matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is described. The phenomenon was observed during the analysis of tryptic peptides from insulin and was confirmed in the determination of disulfide bonds in the rhamnose-binding lectin SEL24K from the Chinook salmon Oncorhynchus tshawytscha. A possible mechanism for this surprising scrambling is proposed. Despite this finding, the disulfide bond pattern in SEL24K was assigned unambiguously by a multi-enzyme digestion strategy in combination with MALDI mass spectrometry. The pattern was found to be symmetrical in the tandem repeat sequence of SEL24K. To the best of our knowledge, this is the first report of disulfide bond scrambling in the gas phase during MALDI-MS analysis. This observation has important ramifications for unambiguous assignment of disulfide bonds.

AB - Evidence for photo-induced radical disulfide bond scrambling in the gas phase during matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is described. The phenomenon was observed during the analysis of tryptic peptides from insulin and was confirmed in the determination of disulfide bonds in the rhamnose-binding lectin SEL24K from the Chinook salmon Oncorhynchus tshawytscha. A possible mechanism for this surprising scrambling is proposed. Despite this finding, the disulfide bond pattern in SEL24K was assigned unambiguously by a multi-enzyme digestion strategy in combination with MALDI mass spectrometry. The pattern was found to be symmetrical in the tandem repeat sequence of SEL24K. To the best of our knowledge, this is the first report of disulfide bond scrambling in the gas phase during MALDI-MS analysis. This observation has important ramifications for unambiguous assignment of disulfide bonds.

UR - http://www.scopus.com/inward/record.url?scp=69549120036&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=69549120036&partnerID=8YFLogxK

U2 - 10.1016/j.jasms.2009.04.021

DO - 10.1016/j.jasms.2009.04.021

M3 - Article

C2 - 19535267

AN - SCOPUS:69549120036

SN - 1044-0305

VL - 20

SP - 1603

EP - 1616

JO - Journal of the American Society for Mass Spectrometry

JF - Journal of the American Society for Mass Spectrometry

IS - 9

ER -

Gas-Phase Scrambling of Disulfide Bonds during Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Analysis

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this