Functional polymorphism in the 5′-UTR of CR2 is associated with susceptibility to nasopharyngeal carcinoma

Qin Fan, Jun Fang He, Qi Rui Wang, Hong Bing Cai, Xue Gang Sun, Xin Xi Zhou, Hai De Qin, Yin Yao Shugart, Wei Hua Jia

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Epstein-Barr virus (EBV)-associated nasopharyngeal carcinoma (NPC) is a squamous cell cancer endemic in Southern China and Southeast Asia. It has been shown that inflammatory and immune responses during EBV infection contribute to the development of NPC. The complement receptor 2 (CR2) gene plays central roles during inflammatory and immune responses and, therefore, is a good candidate susceptibility gene for NPC. We performed PCR-based sequencing to identify multiple single-nucleotide polymorphisms (SNPs) within the exon regions of the CR2 gene in a Cantonese population. Two SNPs were screened in 528 NPC patients and 408 normal individuals to perform a case-control study matched according to age, gender and residence. Furthermore, we cloned the entire 5′-UTR and entire CR2 promoter into a luciferase report system and compared the luciferase activities between the different allelic constructs. A SNP in the 5′-UTR of CR2 (24 T/C, rs3813946) showed a significant association (P45 years. In group 1, the allelic frequencies of 24 T/C in the patients were significantly different from those of the controls (P=0.0034). The odds ratio (OR=1.81) also indicated a higher risk of NPC in individuals who carried the minor allele C. All constructs exerted allelic differences on luciferase activities, but only the susceptible allele +24C construct showed increased activity. Our findings implicate CR2 as a susceptibility gene for NPC and suggest that enhanced CR2 expression may be involved in the oncogenesis and development of NPC.

Original languageEnglish (US)
Pages (from-to)11-16
Number of pages6
JournalOncology Reports
Issue number1
StatePublished - Jul 2013
Externally publishedYes


  • Case-control study
  • Complement receptor 2
  • Nasopharyngeal carcinoma
  • Single-nucleotide polymorphism
  • Susceptibility

ASJC Scopus subject areas

  • Cancer Research
  • Oncology


Dive into the research topics of 'Functional polymorphism in the 5′-UTR of CR2 is associated with susceptibility to nasopharyngeal carcinoma'. Together they form a unique fingerprint.

Cite this