Functional characterization of SgnI: a type II thioesterase critical for natamycin biosynthesis in Streptomyces gilvosporeus

Keyphrases

• Functional Characterization 100%
• Malonyl-CoA 100%
• Type II Thioesterase 100%
• Streptomyces Gilvosporeus 100%
• Natamycin Production 100%
• Thioesterases 80%
• Thioesterase 40%
• Biosynthetic Cluster 40%
• Ethylmalonyl-CoA 40%
• Molecular Mechanism 20%
• Acetyl Coenzyme A (acetyl-CoA) 20%
• Substrate Specificity 20%
• Malonyl 20%
• Kcat 20%
• Natural Products 20%
• Km Value 20%
• High Catalytic Activity 20%
• Steady-state Kinetics 20%
• Substrate Binding 20%
• Wedge Shape 20%
• Protein Folding Intermediates 20%
• Acyl Carrier Protein 20%
• Acyl 20%
• Enhanced Interaction 20%
• Molecular Dynamics Simulation 20%
• Assembly Line 20%
• Binding Residues 20%
• Hydrophobic Substrates 20%
• Structural Modeling 20%
• Structure Feature 20%
• Active Pocket 20%
• Binding Cleft 20%
• Cluster Type 20%
• Phosphopantetheine 20%
• Natamycin 20%
• Methylmalonyl-CoA 20%
• Substrate Size 20%
• Kinetic Features 20%
• Trans-acting 20%
• Modular Polyketide Synthase 20%

Biochemistry, Genetics and Molecular Biology

• Anabolism 100%
• Malonyl-CoA 100%
• Thioesterase 100%
• Streptomyces 100%
• Enzymatic Hydrolysis 40%
• Active Site 40%
• Catalytic Efficiency 40%
• Steady State 20%
• Enzyme Specificity 20%
• Acyl Carrier Protein 20%
• Phosphopantetheine 20%
• Polyketide Synthase 20%
• Acetyl-CoA 20%
• Methylmalonyl-CoA 20%
• Molecular Mechanism 20%