Abstract
The AGPL1 (ADP-glucose pyrophosphorylase large subunit 1) promoter from watermelon (Citrullus vulgaris S.) has proved to exhibit fruit-specific expression patterns in tomato (Lycopersicon esculentum L.). A plant expression vector harboring sweet-taste protein, Brazzein, directed by AGPL1 promoter, was constructed and transferred into tomato plants through Agrobacterium-mediated transform methods. Histochemical staining assay, PCR screening, Southern blotting analysis and RT-PCR analysis showed that Brazzein gene was successfully integrated into the genome of transgenic tomato plants with stable expression. Sweet-taste fruits were produced under control of fruit-specific AGPL1 promoter, whereas other parameters of fruit quality were largely unchanged.
Original language | English (US) |
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Pages (from-to) | 663-667 |
Number of pages | 5 |
Journal | Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji |
Volume | 31 |
Issue number | 6 |
DOIs | |
State | Published - Jun 2009 |
Externally published | Yes |
ASJC Scopus subject areas
- General Medicine