@inproceedings{af47347d76294d0f9a4904d7d6227d1b,
title = "Frequency domain lifetimes of liganded and unliganded hemoglobin measured with a 10 GHz instrument using front face geometry on a free liquid surface",
abstract = "We present data on lifetimes of hemoglobin solutions obtained using a 10 GHz frequency domain fluorometer and a specially designed cuvette which allows front face excitation on a free liquid surface. The cuvette eliminates reflections and stray emissions, which become significant for low intensity fluorophores like hemoglobin. Three lifetimes are detectable in the subnanosecond range. At high frequency it is possible to detect components below 10 ps. The average lifetime of hemoglobin is ligand dependent, opening a new chapter in the investigation of the allosteric behavior of hemoglobin.",
author = "Enrico Bucci and Zygmunt Gryczynsky and Clara Fronticelli and Gabor Laczko and Henryk Malak and Lakowicz, {Joseph R.}",
year = "1990",
language = "English (US)",
isbn = "0819402451",
series = "Proceedings of SPIE - The International Society for Optical Engineering",
publisher = "Publ by Int Soc for Optical Engineering",
pages = "813--819",
editor = "Lakowicz, {Joseph F.}",
booktitle = "Proceedings of SPIE - The International Society for Optical Engineering",
note = "Time-Resolved Laser Spectroscopy in Biochemistry II ; Conference date: 15-01-1990 Through 17-01-1990",
}