Free calcium in heart muscle at rest and during contraction measured with Ca2+-sensitive microelectrodes

Eduardo Marban; Timothy J. Rink; Richard W. Tsien; Roger Y. Tsien

doi:10.1038/286845a0

Free calcium in heart muscle at rest and during contraction measured with Ca²⁺-sensitive microelectrodes

Eduardo Marban, Timothy J. Rink, Richard W. Tsien, Roger Y. Tsien

Research output: Contribution to journal › Article › peer-review

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Abstract

Direct measurements of free Ca²⁺ in heart cells are needed for an understanding of the regulation of contractility. We developed and used Ca²⁺-sensitive microelectrodes with fine tips, stable properties and ample sensitivity to free Ca²⁺ in the sub-micromolar range. In quiescent ventricular muscle, measurements which passed tests for electrode sealing and cell viability gave a mean free Ca²⁺ concentration of 0.26 μM. During contractures, we recorded Ca²⁺ transients rising as high as 10 μM. In studying the effects of catecholamines on free Ca²⁺ and force, we found evidence that adrenaline can reduce myofibrillar Ca²⁺ sensitivity in intact heart muscle.

Original language	English (US)
Pages (from-to)	845-850
Number of pages	6
Journal	Nature
Volume	286
Issue number	5776
DOIs	https://doi.org/10.1038/286845a0
State	Published - 1980
Externally published	Yes

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title = "Free calcium in heart muscle at rest and during contraction measured with Ca2+-sensitive microelectrodes",

abstract = "Direct measurements of free Ca2+ in heart cells are needed for an understanding of the regulation of contractility. We developed and used Ca2+-sensitive microelectrodes with fine tips, stable properties and ample sensitivity to free Ca2+ in the sub-micromolar range. In quiescent ventricular muscle, measurements which passed tests for electrode sealing and cell viability gave a mean free Ca2+ concentration of 0.26 μM. During contractures, we recorded Ca2+ transients rising as high as 10 μM. In studying the effects of catecholamines on free Ca2+ and force, we found evidence that adrenaline can reduce myofibrillar Ca2+ sensitivity in intact heart muscle.",

author = "Eduardo Marban and Rink, {Timothy J.} and Tsien, {Richard W.} and Tsien, {Roger Y.}",

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AU - Tsien, Richard W.

AU - Tsien, Roger Y.

PY - 1980

Y1 - 1980

N2 - Direct measurements of free Ca2+ in heart cells are needed for an understanding of the regulation of contractility. We developed and used Ca2+-sensitive microelectrodes with fine tips, stable properties and ample sensitivity to free Ca2+ in the sub-micromolar range. In quiescent ventricular muscle, measurements which passed tests for electrode sealing and cell viability gave a mean free Ca2+ concentration of 0.26 μM. During contractures, we recorded Ca2+ transients rising as high as 10 μM. In studying the effects of catecholamines on free Ca2+ and force, we found evidence that adrenaline can reduce myofibrillar Ca2+ sensitivity in intact heart muscle.

AB - Direct measurements of free Ca2+ in heart cells are needed for an understanding of the regulation of contractility. We developed and used Ca2+-sensitive microelectrodes with fine tips, stable properties and ample sensitivity to free Ca2+ in the sub-micromolar range. In quiescent ventricular muscle, measurements which passed tests for electrode sealing and cell viability gave a mean free Ca2+ concentration of 0.26 μM. During contractures, we recorded Ca2+ transients rising as high as 10 μM. In studying the effects of catecholamines on free Ca2+ and force, we found evidence that adrenaline can reduce myofibrillar Ca2+ sensitivity in intact heart muscle.

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Free calcium in heart muscle at rest and during contraction measured with Ca²⁺-sensitive microelectrodes

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