Fourier domain pump-probe optical coherence tomography imaging of Melanin

Desmond Jacob, Ryan L. Shelton, Brian E. Applegate

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


We report the development of a two-color Fourier domain Pump-Probe Optical Coherence Tomography (PPOCT) system. Tissue phantom experiments to characterize the system performance demonstrated imaging depths in excess of 725 μm, nearly comparable to the base Optical Coherence Tomography system. PPOCT A-line rates were also demonstrated in excess of 1 kHz. The physical origin of the PPOCT signal was investigated with a series of experiments which revealed that the signal is a mixture of short and long lifetime component signals. The short lifetime component was attributed to transient absorption while the long lifetime component may be due to a mixture of transient absorption and thermal effects. Ex vivo images of porcine iris demonstrated the potential for imaging melanin in the eye, where cancer of the melanocytes is the most common form of eye cancer in adults.

Original languageEnglish (US)
Pages (from-to)12399-12410
Number of pages12
JournalOptics Express
Issue number12
StatePublished - Jun 7 2010
Externally publishedYes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics


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