Four classes of HERV-K long terminal repeats and their relative promoter strengths for transcription

David E. Mold; Tzyy Choou Wu; Frederic Askin; Ru Chih C. Huang

doi:10.1007/BF02255597

Four classes of HERV-K long terminal repeats and their relative promoter strengths for transcription

David E. Mold, Tzyy Choou Wu, Frederic Askin, Ru Chih C. Huang

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

The human genome contains abundant copies of HERV-K endogenous retro- virus sequences. These remnants of ancient infection have the potential to reshape the genome by retrotransposition or infection since they are highly expressed in cells of germ line origin. When conserved nucleotide sequence variations in the U3 region of the HERV-K 3' LTR were used to distinguish between individual HERV-K proviruses, 4 major classes of HERV-K LTRs were identified. HERV-K U3 regions from the different classes were compared in an in vitro transcription assay and were found to vary significantly in their promoter activities.

Original language	English (US)
Pages (from-to)	78-82
Number of pages	5
Journal	Journal of biomedical science
Volume	4
Issue number	2-3
DOIs	https://doi.org/10.1007/BF02255597
State	Published - Mar 1997

Keywords

Human endogenous retrovirus
In vitro transcription
LTR U3 DNA sequence

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Molecular Biology
Clinical Biochemistry
Cell Biology
Biochemistry, medical
Pharmacology (medical)

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10.1007/BF02255597

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abstract = "The human genome contains abundant copies of HERV-K endogenous retro- virus sequences. These remnants of ancient infection have the potential to reshape the genome by retrotransposition or infection since they are highly expressed in cells of germ line origin. When conserved nucleotide sequence variations in the U3 region of the HERV-K 3' LTR were used to distinguish between individual HERV-K proviruses, 4 major classes of HERV-K LTRs were identified. HERV-K U3 regions from the different classes were compared in an in vitro transcription assay and were found to vary significantly in their promoter activities.",

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note = "Funding Information: This work was supported by NIH grants 5R01CH48263 and 3R01AI3230i. We thank Paul Giza, Wen-Nan Tseng and Scott Smith for their assistance with the in vitro transcription assay and Dr. Karen Beernon for the use of the Packard Instant Imager.",

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N1 - Funding Information: This work was supported by NIH grants 5R01CH48263 and 3R01AI3230i. We thank Paul Giza, Wen-Nan Tseng and Scott Smith for their assistance with the in vitro transcription assay and Dr. Karen Beernon for the use of the Packard Instant Imager.

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AB - The human genome contains abundant copies of HERV-K endogenous retro- virus sequences. These remnants of ancient infection have the potential to reshape the genome by retrotransposition or infection since they are highly expressed in cells of germ line origin. When conserved nucleotide sequence variations in the U3 region of the HERV-K 3' LTR were used to distinguish between individual HERV-K proviruses, 4 major classes of HERV-K LTRs were identified. HERV-K U3 regions from the different classes were compared in an in vitro transcription assay and were found to vary significantly in their promoter activities.

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Four classes of HERV-K long terminal repeats and their relative promoter strengths for transcription

Abstract

Keywords

ASJC Scopus subject areas

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