TY - JOUR
T1 - Formation of reactive oxygen species and of 8-hydroxy-2'-deoxyguanosine in DNA in vitro with betel-quid ingredients.
AU - Friesen, M.
AU - Maru, G.
AU - Bussachini, V.
AU - Bartsch, H.
AU - Nair, U.
AU - Nair, J.
AU - Floyd, R. A.
PY - 1988
Y1 - 1988
N2 - Using a chemiluminescence technique, superoxide anion (O2-.) and H2O2 were shown to be formed in vitro, above pH 9.5, from betel-quid (BQ) ingredients, such as areca-nut extract and catechu. The formation of O2-. was enhanced by Fe2+, Fe3+ and Cu2+ and inhibited by Mn2+. Saliva was found to inhibit both O2-. and H2O2 formation from BQ ingredients. Upon incubation of DNA at alkaline pH with areca-nut extract or catechu, in the presence or absence of Fe3+, 8-hydroxy-2'-deoxyguanosine was formed, as quantified by high-performance liquid chromatography. The data suggest a possible role of reactive oxygen species (ROS) in the etiology of oral cancer in betel quid chewers.
AB - Using a chemiluminescence technique, superoxide anion (O2-.) and H2O2 were shown to be formed in vitro, above pH 9.5, from betel-quid (BQ) ingredients, such as areca-nut extract and catechu. The formation of O2-. was enhanced by Fe2+, Fe3+ and Cu2+ and inhibited by Mn2+. Saliva was found to inhibit both O2-. and H2O2 formation from BQ ingredients. Upon incubation of DNA at alkaline pH with areca-nut extract or catechu, in the presence or absence of Fe3+, 8-hydroxy-2'-deoxyguanosine was formed, as quantified by high-performance liquid chromatography. The data suggest a possible role of reactive oxygen species (ROS) in the etiology of oral cancer in betel quid chewers.
UR - http://www.scopus.com/inward/record.url?scp=0024196022&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024196022&partnerID=8YFLogxK
M3 - Article
C2 - 2848765
AN - SCOPUS:0024196022
SN - 0300-5038
SP - 417
EP - 421
JO - IARC scientific publications
JF - IARC scientific publications
IS - 89
ER -