Abstract
This work reports the intracellular pH (pH(i)) dynamics of Saccharomyces cerevisiae cells in sporulation medium. Cells loaded with the pH-sensitive dye carboxy-seminaphthorhodafluor-1 (C.SNARF-1) exhibited an alkalization of the pH(i) following the extracellular pH during sporulation in the absence of buffer and almost no change in pH(i) or ΔpH when sporulation was carried out in buffered medium. The results indicate that the pH gradient does not appear to be directly involved in the regulation of acetate uptake during sporulation. However, the alkalization of pH(i) by eliciting a decrease in metabolic fluxes could account for a lower demand for acetate.
Original language | English (US) |
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Pages (from-to) | 17-23 |
Number of pages | 7 |
Journal | FEMS microbiology letters |
Volume | 153 |
Issue number | 1 |
DOIs | |
State | Published - Aug 1 1997 |
Keywords
- Fluorescent dye
- Intracellular pH
- Saccharomyces cerevisiae
- Sporulation
ASJC Scopus subject areas
- Microbiology
- Molecular Biology
- Genetics