@inproceedings{4deb7616ea1b4255bcb5800329a89ce6,
title = "Fluorescence intensity and anisotropy decays of the intrinsic tryptophan emission of hemoglobin measured with a 10-GHz fluorometer using front-face geometry on free liquid surface",
abstract = "We measure the intensity and anisotropy decays of the intrinsic tryptophan emission from hemoglobin solutions obtained using a 10 GHz frequency-domain fluorometer and a specially designed cuvette which allows front face excitation on a free liquid surface. The cuvette eliminates reflections and stray emissions, which become significant for low intensity fluorescence like in hemoglobin. Three lifetimes are detectable in the subnanosecond range. The average lifetime of hemoglobin is ligand dependent. Fluorescence anisotropy decays of oxy, deoxy, and carbonmonoxyhemoglobin can be fitted with up to three correlation times. When three components are used the floating initial anisotropy ro is in each case higher than the steady-state anisotropy of tryptophan in vitrified solution. For deoxy hemoglobin it is close to 0.4. The data are consistent with an initial loss of anisotropy from 0.4 to about 0.2 occurring in the first two picoseconds.",
author = "Enrico Bucci and Zygmunt Gryczynski and Clara Fronticelli and Ignacy Gryczynski and Lakowicz, {Joseph R.}",
year = "1992",
month = jan,
day = "1",
language = "English (US)",
isbn = "0819407860",
series = "Proceedings of SPIE - The International Society for Optical Engineering",
publisher = "Publ by Int Soc for Optical Engineering",
pages = "556--561",
booktitle = "Proceedings of SPIE - The International Society for Optical Engineering",
note = "Time-Resolved Laser Spectroscopy in Biochemistry III ; Conference date: 20-01-1992 Through 22-01-1992",
}