Fluorescence in situ hybridization to determine engraftment status after murine bone marrow transplant

Anita L. Hawkins; Richard J. Jones; Barbara A. Zehnbauer; Milada S. Zicha; Michael J. Collector; Saul J. Sharkis; Constance A. Griffin

doi:10.1016/0165-4608(92)90345-9

Fluorescence in situ hybridization to determine engraftment status after murine bone marrow transplant

Anita L. Hawkins
, Richard J. Jones
, Barbara A. Zehnbauer
, Milada S. Zicha
, Michael J. Collector
, Saul J. Sharkis
, Constance A. Griffin

School of Medicine

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

The mouse Y-specific DNA sequence pY2 was used as a probe for fluorescence in situ hybridization (FISH) to evaluate murine hematopoietic tissues after sex-mismatched bone marrow transplant (BMT). The pY2 probe was localized to the long arm of the Y chromosome on BM metaphases. Hybridization of pY2 in FISH of interphase cells from BM, spleen, and thymus after BMT was compared with Southern blot analysis; both methods gave comparable results. Only FISH was able to analyze post-BMT peripheral blood (PB) samples successfully, and provides a useful method for following engraftment status in the mouse on an ongoing basis.

Original language	English (US)
Pages (from-to)	145-148
Number of pages	4
Journal	Cancer Genetics and Cytogenetics
Volume	64
Issue number	2
DOIs	https://doi.org/10.1016/0165-4608(92)90345-9
State	Published - Dec 1992

ASJC Scopus subject areas

Molecular Biology
Genetics
Cancer Research

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10.1016/0165-4608(92)90345-9

Cite this

@article{c0acab2ed5e7496a9b83ae35383ac446,

title = "Fluorescence in situ hybridization to determine engraftment status after murine bone marrow transplant",

abstract = "The mouse Y-specific DNA sequence pY2 was used as a probe for fluorescence in situ hybridization (FISH) to evaluate murine hematopoietic tissues after sex-mismatched bone marrow transplant (BMT). The pY2 probe was localized to the long arm of the Y chromosome on BM metaphases. Hybridization of pY2 in FISH of interphase cells from BM, spleen, and thymus after BMT was compared with Southern blot analysis; both methods gave comparable results. Only FISH was able to analyze post-BMT peripheral blood (PB) samples successfully, and provides a useful method for following engraftment status in the mouse on an ongoing basis.",

author = "Hawkins, \{Anita L.\} and Jones, \{Richard J.\} and Zehnbauer, \{Barbara A.\} and Zicha, \{Milada S.\} and Collector, \{Michael J.\} and Sharkis, \{Saul J.\} and Griffin, \{Constance A.\}",

note = "Funding Information: This work was partially funded by NIH Grants No. 1R01-HL46533-01 (to R.J.J. and S.J.S.) and No. 5POI-CA15396-17 (to A.L.H. and C.A.G.).",

year = "1992",

month = dec,

doi = "10.1016/0165-4608(92)90345-9",

language = "English (US)",

volume = "64",

pages = "145--148",

journal = "Cancer Genetics and Cytogenetics",

issn = "0165-4608",

publisher = "Elsevier Inc.",

number = "2",

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TY - JOUR

T1 - Fluorescence in situ hybridization to determine engraftment status after murine bone marrow transplant

AU - Hawkins, Anita L.

AU - Jones, Richard J.

AU - Zehnbauer, Barbara A.

AU - Zicha, Milada S.

AU - Collector, Michael J.

AU - Sharkis, Saul J.

AU - Griffin, Constance A.

N1 - Funding Information: This work was partially funded by NIH Grants No. 1R01-HL46533-01 (to R.J.J. and S.J.S.) and No. 5POI-CA15396-17 (to A.L.H. and C.A.G.).

PY - 1992/12

Y1 - 1992/12

N2 - The mouse Y-specific DNA sequence pY2 was used as a probe for fluorescence in situ hybridization (FISH) to evaluate murine hematopoietic tissues after sex-mismatched bone marrow transplant (BMT). The pY2 probe was localized to the long arm of the Y chromosome on BM metaphases. Hybridization of pY2 in FISH of interphase cells from BM, spleen, and thymus after BMT was compared with Southern blot analysis; both methods gave comparable results. Only FISH was able to analyze post-BMT peripheral blood (PB) samples successfully, and provides a useful method for following engraftment status in the mouse on an ongoing basis.

AB - The mouse Y-specific DNA sequence pY2 was used as a probe for fluorescence in situ hybridization (FISH) to evaluate murine hematopoietic tissues after sex-mismatched bone marrow transplant (BMT). The pY2 probe was localized to the long arm of the Y chromosome on BM metaphases. Hybridization of pY2 in FISH of interphase cells from BM, spleen, and thymus after BMT was compared with Southern blot analysis; both methods gave comparable results. Only FISH was able to analyze post-BMT peripheral blood (PB) samples successfully, and provides a useful method for following engraftment status in the mouse on an ongoing basis.

UR - https://www.scopus.com/pages/publications/0027057660

UR - https://www.scopus.com/pages/publications/0027057660#tab=citedBy

U2 - 10.1016/0165-4608(92)90345-9

DO - 10.1016/0165-4608(92)90345-9

M3 - Article

C2 - 1486564

AN - SCOPUS:0027057660

SN - 0165-4608

VL - 64

SP - 145

EP - 148

JO - Cancer Genetics and Cytogenetics

JF - Cancer Genetics and Cytogenetics

IS - 2

ER -

Fluorescence in situ hybridization to determine engraftment status after murine bone marrow transplant

Abstract

ASJC Scopus subject areas

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