Flow modulates endothelial regulation of smooth muscle cell proliferation: A new model

G. B. Nackman; M. F. Fillinger; R. Shafritz; T. Wei; A. M. Graham; J. A. Freischlag; K. C. Kent

doi:10.1016/S0039-6060(98)70141-2

Flow modulates endothelial regulation of smooth muscle cell proliferation: A new model

G. B. Nackman, M. F. Fillinger, R. Shafritz, T. Wei, A. M. Graham, J. A. Freischlag, K. C. Kent

Research output: Contribution to journal › Article › peer-review

49 Scopus citations

Abstract

Background. With a co-culture model, we have previously demonstrated that endothelial cells (ECs) exert regulatory control over smooth muscle cell (SMC) behavior. ECs appeared to stimulate SMC proliferation in static culture. This Study was performed to test the hypothesis that the EC stimulation of SMC proliferation was effected by shear stress. Methods. Bovine SMCs were cultured on a thin semipermeable membrane either alone or opposite ECs in co-culture (SMC/EC). A novel parallel-plate flow device was developed and used for exposing the EC side of the co-culture to shear stress. EC and SMC proliferation rates were determined after 24 hours' exposure to 0, 1, or 10 dynes/cm² of shear stress. Results. SMC proliferation decreased significantly from 362 ± 65 cpm/μg DNA (control, mean ± SEM) to 68 ± 43 cpm/μg (1 dyne/cm²) and 99 ± 18 cpm/μg (10 dynes/cm²) (P <. 05). EC proliferation after flow decreased as compared with no-flow controls 71 ± 15 cpm/μg DNA (control, mean ± SEM) to 29 ± 5 cpm/μg (1 dyne/cm²) and 21 ± 4 cpm/μg (10 dynes/cm²) (P < .05). Conclusions. In a model designed to study SMC/EC interactions in a flow environment, it was seen that EC exposure to shear stress alters the growth characteristics of SMCs. This suggests that hemodynamic mechanical forces may be sufficient to alter the EC regulation of SMC behavior.

Original language	English (US)
Pages (from-to)	353-361
Number of pages	9
Journal	Surgery
Volume	124
Issue number	2
DOIs	https://doi.org/10.1016/S0039-6060(98)70141-2
State	Published - 1998
Externally published	Yes

ASJC Scopus subject areas

Surgery

Access to Document

10.1016/S0039-6060(98)70141-2

Cite this

@article{b9bff5eda9de4e04a321e4af0bd70f93,

title = "Flow modulates endothelial regulation of smooth muscle cell proliferation: A new model",

abstract = "Background. With a co-culture model, we have previously demonstrated that endothelial cells (ECs) exert regulatory control over smooth muscle cell (SMC) behavior. ECs appeared to stimulate SMC proliferation in static culture. This Study was performed to test the hypothesis that the EC stimulation of SMC proliferation was effected by shear stress. Methods. Bovine SMCs were cultured on a thin semipermeable membrane either alone or opposite ECs in co-culture (SMC/EC). A novel parallel-plate flow device was developed and used for exposing the EC side of the co-culture to shear stress. EC and SMC proliferation rates were determined after 24 hours' exposure to 0, 1, or 10 dynes/cm2 of shear stress. Results. SMC proliferation decreased significantly from 362 ± 65 cpm/μg DNA (control, mean ± SEM) to 68 ± 43 cpm/μg (1 dyne/cm2) and 99 ± 18 cpm/μg (10 dynes/cm2) (P <. 05). EC proliferation after flow decreased as compared with no-flow controls 71 ± 15 cpm/μg DNA (control, mean ± SEM) to 29 ± 5 cpm/μg (1 dyne/cm2) and 21 ± 4 cpm/μg (10 dynes/cm2) (P < .05). Conclusions. In a model designed to study SMC/EC interactions in a flow environment, it was seen that EC exposure to shear stress alters the growth characteristics of SMCs. This suggests that hemodynamic mechanical forces may be sufficient to alter the EC regulation of SMC behavior.",

author = "Nackman, {G. B.} and Fillinger, {M. F.} and R. Shafritz and T. Wei and Graham, {A. M.} and Freischlag, {J. A.} and Kent, {K. C.}",

note = "Funding Information: Supported by the Foundation of the University of Medicine and Dentistry of New Jersey. ",

year = "1998",

doi = "10.1016/S0039-6060(98)70141-2",

language = "English (US)",

volume = "124",

pages = "353--361",

journal = "Surgery",

issn = "0039-6060",

publisher = "Elsevier Inc.",

number = "2",

}

TY - JOUR

T1 - Flow modulates endothelial regulation of smooth muscle cell proliferation

T2 - A new model

AU - Nackman, G. B.

AU - Fillinger, M. F.

AU - Shafritz, R.

AU - Wei, T.

AU - Graham, A. M.

AU - Freischlag, J. A.

AU - Kent, K. C.

N1 - Funding Information: Supported by the Foundation of the University of Medicine and Dentistry of New Jersey.

PY - 1998

Y1 - 1998

N2 - Background. With a co-culture model, we have previously demonstrated that endothelial cells (ECs) exert regulatory control over smooth muscle cell (SMC) behavior. ECs appeared to stimulate SMC proliferation in static culture. This Study was performed to test the hypothesis that the EC stimulation of SMC proliferation was effected by shear stress. Methods. Bovine SMCs were cultured on a thin semipermeable membrane either alone or opposite ECs in co-culture (SMC/EC). A novel parallel-plate flow device was developed and used for exposing the EC side of the co-culture to shear stress. EC and SMC proliferation rates were determined after 24 hours' exposure to 0, 1, or 10 dynes/cm2 of shear stress. Results. SMC proliferation decreased significantly from 362 ± 65 cpm/μg DNA (control, mean ± SEM) to 68 ± 43 cpm/μg (1 dyne/cm2) and 99 ± 18 cpm/μg (10 dynes/cm2) (P <. 05). EC proliferation after flow decreased as compared with no-flow controls 71 ± 15 cpm/μg DNA (control, mean ± SEM) to 29 ± 5 cpm/μg (1 dyne/cm2) and 21 ± 4 cpm/μg (10 dynes/cm2) (P < .05). Conclusions. In a model designed to study SMC/EC interactions in a flow environment, it was seen that EC exposure to shear stress alters the growth characteristics of SMCs. This suggests that hemodynamic mechanical forces may be sufficient to alter the EC regulation of SMC behavior.

AB - Background. With a co-culture model, we have previously demonstrated that endothelial cells (ECs) exert regulatory control over smooth muscle cell (SMC) behavior. ECs appeared to stimulate SMC proliferation in static culture. This Study was performed to test the hypothesis that the EC stimulation of SMC proliferation was effected by shear stress. Methods. Bovine SMCs were cultured on a thin semipermeable membrane either alone or opposite ECs in co-culture (SMC/EC). A novel parallel-plate flow device was developed and used for exposing the EC side of the co-culture to shear stress. EC and SMC proliferation rates were determined after 24 hours' exposure to 0, 1, or 10 dynes/cm2 of shear stress. Results. SMC proliferation decreased significantly from 362 ± 65 cpm/μg DNA (control, mean ± SEM) to 68 ± 43 cpm/μg (1 dyne/cm2) and 99 ± 18 cpm/μg (10 dynes/cm2) (P <. 05). EC proliferation after flow decreased as compared with no-flow controls 71 ± 15 cpm/μg DNA (control, mean ± SEM) to 29 ± 5 cpm/μg (1 dyne/cm2) and 21 ± 4 cpm/μg (10 dynes/cm2) (P < .05). Conclusions. In a model designed to study SMC/EC interactions in a flow environment, it was seen that EC exposure to shear stress alters the growth characteristics of SMCs. This suggests that hemodynamic mechanical forces may be sufficient to alter the EC regulation of SMC behavior.

UR - http://www.scopus.com/inward/record.url?scp=0031875473&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031875473&partnerID=8YFLogxK

U2 - 10.1016/S0039-6060(98)70141-2

DO - 10.1016/S0039-6060(98)70141-2

M3 - Article

C2 - 9706159

AN - SCOPUS:0031875473

SN - 0039-6060

VL - 124

SP - 353

EP - 361

JO - Surgery

JF - Surgery

IS - 2

ER -

Flow modulates endothelial regulation of smooth muscle cell proliferation: A new model

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this