TY - JOUR
T1 - Five SWI genes are required for expression of the HO gene in yeast
AU - Stern, Michael
AU - Jensen, Robert
AU - Herskowitz, Ira
N1 - Funding Information:
11-r thank Buff Blair for constructing the parent strain and suggwting the mutant spawn. (:. Kohlhaw for helpful disc.ussions. Stan Fields. ,IJikr Hall. Sandy Johnson, .lo Ann Cl’iw anti Ken Zaret for c~omtnrnts on the manuscript. John Gatnr and \‘incr Groppi f’ot c.otnmunivating results prior to publication and Jim Haher for supplying his xwi mutant. Thr rrsrarch was supported by research grant AI-13162 from the Public Health Servirr. M.S. was the rwipient of a Sational Scirnw Foundation Graduatr Fellowship and a Yttivrrsit; of (‘alifornia (Ihanwllor’s Fellowship. R.J. was the rrcipient of a Public Health Srrviw hationitl Rrsrarrh Srrviw .Iward in (:twetics to thr I)rpartmtvt of Kinlop? (I’nivrrsity of (hqon).
PY - 1984/10/5
Y1 - 1984/10/5
N2 - High-frequency mating type interconversion in yeast requires the HO gene, which encodes a site-specific endonuclease that initiates the switching process. We have isolated and analyzed switching-defective mutants. These mutants define five complementation and linkage groups, SWI 1 to SWI 5. We have shown by two assays, Northern hybridization and β-galactosidase activity in strains containing an HO-lacZ fusion, that mutants defective any SWI gene fail to express the HO gene. In addition, all of the swi mutants exhibit other phenotypes. the most notable being the inviability of double mutants defective in SWI 4 and in either SWI 1, SWI 2 or SWI 3. These results indicate that the SWI genes function in some way as positive regulators of HO expression and have additional cellular roles.
AB - High-frequency mating type interconversion in yeast requires the HO gene, which encodes a site-specific endonuclease that initiates the switching process. We have isolated and analyzed switching-defective mutants. These mutants define five complementation and linkage groups, SWI 1 to SWI 5. We have shown by two assays, Northern hybridization and β-galactosidase activity in strains containing an HO-lacZ fusion, that mutants defective any SWI gene fail to express the HO gene. In addition, all of the swi mutants exhibit other phenotypes. the most notable being the inviability of double mutants defective in SWI 4 and in either SWI 1, SWI 2 or SWI 3. These results indicate that the SWI genes function in some way as positive regulators of HO expression and have additional cellular roles.
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U2 - 10.1016/0022-2836(84)90315-2
DO - 10.1016/0022-2836(84)90315-2
M3 - Article
C2 - 6436497
AN - SCOPUS:0021659727
SN - 0022-2836
VL - 178
SP - 853
EP - 868
JO - Journal of molecular biology
JF - Journal of molecular biology
IS - 4
ER -