First report of Enterocytozoon bieneusi infection on a pig farm in the Czech Republic

Bohumil Sak, Martin Kváč, Dagmar Hanzlíková, Vitaliano Cama

Research output: Contribution to journalArticlepeer-review

68 Scopus citations


Enterocytozoon bieneusi infects humans and animals and can cause life-threatening diarrhea in immunocompromised people. The routes of transmission and its zoonotic potential are not fully understood. Pigs have been frequently reported to have E. bieneusi; therefore, we surveyed farm-raised pigs in the Czech Republic to determine its presence and genetic diversity. Spores were detected by microscopy in the faeces of 65 out of 79 examined animals (82%). A species-specific polymerase chain reaction (PCR) identified E. bieneusi in 94% of samples. Genotyping based on the ITS regions of the SSU rRNA gene identified that most pigs were infected with the species-specific genotype F, while two animals had the zoonotic genotype D and two had genotype Peru 9. This is the first report of E. bieneusi in swine in the Czech Republic, and demonstrated that most infections were with pig-specific genotypes. Nonetheless, swine may still play a role in the transmission of E. bieneusi to humans.

Original languageEnglish (US)
Pages (from-to)220-224
Number of pages5
JournalVeterinary Parasitology
Issue number3-4
StatePublished - May 31 2008


  • Enterocytozoon bieneusi
  • Genotypes
  • Microsporidia
  • Pigs
  • Zoonosis

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Parasitology
  • veterinary(all)


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