Fight against background noise in stimulated emission depletion nanoscopy

Ye Ma, Taekjip Ha

Research output: Contribution to journalReview articlepeer-review

9 Scopus citations


STimulated emission depletion (STED) nanoscopy has been proposed to extend greatly our capability of using light to study a variety of biological problems with nanometer-scale resolution. However, in practice the unwanted background noise degrades the STED image quality and precludes quantitative analysis. Here, we discuss the underlying sources of the background noise in STED images, and review current approaches to alleviate this problem, such as time-gating, anti- Stokes excitation removal, and off-focus incomplete depletion suppression. Progress in correcting uncorrelated background photons in fluorescence correlation spectroscopy combined with STED (STED-FCS) will also be discussed.

Original languageEnglish (US)
Article number051002
JournalPhysical biology
Issue number5
StatePublished - Sep 2019


  • Background noise
  • Optical microscopy
  • STED
  • Superresolution

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Molecular Biology
  • Cell Biology


Dive into the research topics of 'Fight against background noise in stimulated emission depletion nanoscopy'. Together they form a unique fingerprint.

Cite this