Fibronectin mediates mesendodermal cell fate decisions

Paul Cheng, Peter Andersen, David Hassel, Bogac L. Kaynak, Pattraranee Limphong, Lonny Juergensen, Chulan Kwon, Deepak Srivastava

Research output: Contribution to journalArticlepeer-review

42 Scopus citations


Non-cell-autonomous signals often play crucial roles in cell fate decisions during animal development. Reciprocal signaling between endoderm and mesoderm is vital for embryonic development, yet the key signals and mechanisms remain unclear. Here, we show that endodermal cells efficiently promote the emergence of mesodermal cells in the neighboring population through signals containing an essential short-range component. The endoderm-mesoderm interaction promoted precardiac mesoderm formation in mouse embryonic stem cells and involved endodermal production of fibronectin. In vivo, fibronectin deficiency resulted in a dramatic reduction of mesoderm accompanied by endodermal expansion in zebrafish embryos. This event was mediated by regulation of Wnt signaling in mesodermal cells through activation of integrin-β1. Our findings highlight the importance of the extracellular matrix in mediating short-range signals and reveal a novel function of endoderm, involving fibronectin and its downstream signaling cascades, in promoting the emergence of mesoderm.

Original languageEnglish (US)
Pages (from-to)2587-2596
Number of pages10
JournalDevelopment (Cambridge)
Issue number12
StatePublished - Jun 15 2013


  • Endoderm
  • Fibronectin
  • Integrin-β1
  • Mesoderm
  • Wnt

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology


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