FGFR3 expression in primary and metastatic urothelial carcinoma of the bladder

Elizabeth A. Guancial; Lillian Werner; Joaquim Bellmunt; Aristotle Bamias; Toni K. Choueiri; Robert Ross; Fabio A. Schutz; Rachel S. Park; Robert J. O'Brien; Michelle S. Hirsch; Justine A. Barletta; David M. Berman; Rosina Lis; Massimo Loda; Edward C. Stack; Levi A. Garraway; Markus Riester; Franziska Michor; Philip W. Kantoff; Jonathan E. Rosenberg

doi:10.1002/cam4.262

FGFR3 expression in primary and metastatic urothelial carcinoma of the bladder

Elizabeth A. Guancial, Lillian Werner, Joaquim Bellmunt, Aristotle Bamias, Toni K. Choueiri, Robert Ross, Fabio A. Schutz, Rachel S. Park, Robert J. O'Brien, Michelle S. Hirsch, Justine A. Barletta, David M. Berman, Rosina Lis, Massimo Loda, Edward C. Stack, Levi A. Garraway, Markus Riester, Franziska Michor, Philip W. Kantoff, Jonathan E. Rosenberg

Research output: Contribution to journal › Article › peer-review

60 Scopus citations

Abstract

While fibroblast growth factor receptor 3 (FGFR3) is frequently mutated or overexpressed in nonmuscle-invasive urothelial carcinoma (UC), the prevalence of FGFR3 protein expression and mutation remains unknown in muscle-invasive disease. FGFR3 protein and mRNA expression, mutational status, and copy number variation were retrospectively analyzed in 231 patients with formalin-fixed paraffin-embedded primary UCs, 33 metastases, and 14 paired primary and metastatic tumors using the following methods: immunohistochemistry, NanoString nCounterTM, OncoMap or Affymetrix OncoScanTM array, and Gain and Loss of Analysis of DNA and Genomic Identification of Significant Targets in Cancer software. FGFR3 immunohistochemistry staining was present in 29% of primary UCs and 49% of metastases and did not impact overall survival (P = 0.89, primary tumors; P = 0.78, metastases). FGFR3 mutations were observed in 2% of primary tumors and 9% of metastases. Mutant tumors expressed higher levels of FGFR3 mRNA than wild-type tumors (P

Original language	English (US)
Pages (from-to)	835-844
Number of pages	10
Journal	Cancer Medicine
Volume	3
Issue number	4
DOIs	https://doi.org/10.1002/cam4.262
State	Published - 2014
Externally published	Yes

Keywords

Biomarker
Bladder cancer
FGFR3
Metastatic urothelial carcinoma
Muscle-invasive urothelial carcinoma
Targeted therapy

ASJC Scopus subject areas

Cancer Research
Oncology
Radiology Nuclear Medicine and imaging
Medicine(all)

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Guancial, E. A., Werner, L., Bellmunt, J., Bamias, A., Choueiri, T. K., Ross, R., Schutz, F. A., Park, R. S., O'Brien, R. J., Hirsch, M. S., Barletta, J. A., Berman, D. M., Lis, R., Loda, M., Stack, E. C., Garraway, L. A., Riester, M., Michor, F., Kantoff, P. W., & Rosenberg, J. E. (2014). FGFR3 expression in primary and metastatic urothelial carcinoma of the bladder. Cancer Medicine, 3(4), 835-844. https://doi.org/10.1002/cam4.262

Guancial, EA, Werner, L, Bellmunt, J, Bamias, A, Choueiri, TK, Ross, R, Schutz, FA, Park, RS, O'Brien, RJ, Hirsch, MS, Barletta, JA, Berman, DM, Lis, R, Loda, M, Stack, EC, Garraway, LA, Riester, M, Michor, F, Kantoff, PW & Rosenberg, JE 2014, 'FGFR3 expression in primary and metastatic urothelial carcinoma of the bladder', Cancer Medicine, vol. 3, no. 4, pp. 835-844. https://doi.org/10.1002/cam4.262

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abstract = "While fibroblast growth factor receptor 3 (FGFR3) is frequently mutated or overexpressed in nonmuscle-invasive urothelial carcinoma (UC), the prevalence of FGFR3 protein expression and mutation remains unknown in muscle-invasive disease. FGFR3 protein and mRNA expression, mutational status, and copy number variation were retrospectively analyzed in 231 patients with formalin-fixed paraffin-embedded primary UCs, 33 metastases, and 14 paired primary and metastatic tumors using the following methods: immunohistochemistry, NanoString nCounterTM, OncoMap or Affymetrix OncoScanTM array, and Gain and Loss of Analysis of DNA and Genomic Identification of Significant Targets in Cancer software. FGFR3 immunohistochemistry staining was present in 29% of primary UCs and 49% of metastases and did not impact overall survival (P = 0.89, primary tumors; P = 0.78, metastases). FGFR3 mutations were observed in 2% of primary tumors and 9% of metastases. Mutant tumors expressed higher levels of FGFR3 mRNA than wild-type tumors (P ",

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AU - Guancial, Elizabeth A.

AU - Werner, Lillian

AU - Bellmunt, Joaquim

AU - Bamias, Aristotle

AU - Choueiri, Toni K.

AU - Ross, Robert

AU - Schutz, Fabio A.

AU - Park, Rachel S.

AU - O'Brien, Robert J.

AU - Hirsch, Michelle S.

AU - Barletta, Justine A.

AU - Berman, David M.

AU - Lis, Rosina

AU - Loda, Massimo

AU - Stack, Edward C.

AU - Garraway, Levi A.

AU - Riester, Markus

AU - Michor, Franziska

AU - Kantoff, Philip W.

AU - Rosenberg, Jonathan E.

PY - 2014

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FGFR3 expression in primary and metastatic urothelial carcinoma of the bladder

Abstract

Keywords

ASJC Scopus subject areas

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