Fertilization and in vitro development of cryopreserved human prophase I oocytes

T. L. Toth, S. G. Baka, L. L. Veeck, H. W. Jones, S. Muasher, S. E. Lanzendorf

Research output: Contribution to journalArticlepeer-review

116 Scopus citations


Objective: To determine the potential for in vitro maturation, fertilization, and cleavage after cryopreservation of immature, prophase I human oocytes. Design: Immature oocytes obtained in excess of the number required by the patient were randomized and cryopreserved at the prophase I stage or cultured as control. After thawing and maturation in vitro, test and control oocytes were inseminated with husband's sperm and evaluated for fertilization and cleavage in vitro. Setting: In vitro fertilization program. Patients: Consenting patients undergoing controlled ovarian hyperstimulation for the purposes of IVF. Main Outcome Measures: Rates of maturation to metaphase II, fertilization, and cleavage were compared between control and cryopreserved oocytes. Results: Upon thaw, 58.5% (72/123) of prophase I oocytes were viable. Control oocytes demonstrated a 74.8% (98/131) maturation rate to metaphase II, a 56.5% (52/92) fertilization rate, and an 11.5% (6/52) blastocyst rate. Cryopreserved oocytes showed a 83.3% (60/72) rate of maturation, a 57.7% (30/52) fertilization rate, and a 3.3% (1/30) blastocyst rate. No significant differences were noted between any of these parameters. Conclusions: These results demonstrate that prophase I oocytes from stimulated IVF cycles are able to survive cryopreservation and resume meiosis to achieve full nuclear maturation post-thaw. In addition, cryopreserved oocytes retain the same capacity for fertilization and development as control oocytes.

Original languageEnglish (US)
Pages (from-to)891-894
Number of pages4
JournalFertility and sterility
Issue number5
StatePublished - 1994
Externally publishedYes


  • Cryopreservation
  • fertilization
  • in vitro fertilization
  • prophase I oocytes

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology


Dive into the research topics of 'Fertilization and in vitro development of cryopreserved human prophase I oocytes'. Together they form a unique fingerprint.

Cite this