Fast multicolor 3D imaging using aberration-corrected multifocus microscopy

Sara Abrahamsson; Jiji Chen; Bassam Hajj; Sjoerd Stallinga; Alexander Y. Katsov; Jan Wisniewski; Gaku Mizuguchi; Pierre Soule; Florian Mueller; Claire Dugast Darzacq; Xavier Darzacq; Carl Wu; Cornelia I. Bargmann; David A. Agard; Maxime Dahan; Mats G.L. Gustafsson

doi:10.1038/NMETH.2277

Fast multicolor 3D imaging using aberration-corrected multifocus microscopy

Sara Abrahamsson
, Jiji Chen
, Bassam Hajj
, Sjoerd Stallinga
, Alexander Y. Katsov
, Jan Wisniewski
, Gaku Mizuguchi
, Pierre Soule
, Florian Mueller
, Claire Dugast Darzacq
, Xavier Darzacq
, Carl Wu
, Cornelia I. Bargmann
, David A. Agard
, Maxime Dahan
, Mats G.L. Gustafsson

Research output: Contribution to journal › Article › peer-review

Abstract

Conventional acquisition of three-dimensional (3D) microscopy data requires sequential z scanning and is often too slow to capture biological events. We report an aberration-corrected multifocus microscopy method capable of producing an instant focal stack of nine 2D images. Appended to an epifluorescence microscope, the multifocus system enables high-resolution 3D imaging in multiple colors with single-molecule sensitivity, at speeds limited by the camera readout time of a single image.

Original language	English (US)
Pages (from-to)	60-63
Number of pages	4
Journal	Nature Methods
Volume	10
Issue number	1
DOIs	https://doi.org/10.1038/NMETH.2277
State	Published - Jan 2013
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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10.1038/NMETH.2277

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Abrahamsson, S., Chen, J., Hajj, B., Stallinga, S., Katsov, A. Y., Wisniewski, J., Mizuguchi, G., Soule, P., Mueller, F., Darzacq, C. D., Darzacq, X., Wu, C., Bargmann, C. I., Agard, D. A., Dahan, M., & Gustafsson, M. G. L. (2013). Fast multicolor 3D imaging using aberration-corrected multifocus microscopy. Nature Methods, 10(1), 60-63. https://doi.org/10.1038/NMETH.2277

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title = "Fast multicolor 3D imaging using aberration-corrected multifocus microscopy",

abstract = "Conventional acquisition of three-dimensional (3D) microscopy data requires sequential z scanning and is often too slow to capture biological events. We report an aberration-corrected multifocus microscopy method capable of producing an instant focal stack of nine 2D images. Appended to an epifluorescence microscope, the multifocus system enables high-resolution 3D imaging in multiple colors with single-molecule sensitivity, at speeds limited by the camera readout time of a single image.",

author = "Sara Abrahamsson and Jiji Chen and Bassam Hajj and Sjoerd Stallinga and Katsov, \{Alexander Y.\} and Jan Wisniewski and Gaku Mizuguchi and Pierre Soule and Florian Mueller and Darzacq, \{Claire Dugast\} and Xavier Darzacq and Carl Wu and Bargmann, \{Cornelia I.\} and Agard, \{David A.\} and Maxime Dahan and Gustafsson, \{Mats G.L.\}",

note = "Funding Information: ensuring continued funding for this project during and after the difficult time of M.G.{\textquoteright}s sickness. A.Y.K. is supported by a Jane Coffin Childs postdoctoral fellowship. M.D. acknowledges the support of a Fulbright fellowship. X.D. and M.D. are supported by grant ANR-08-PCVI-0013 from Agence Nationale pour la Recherche. D.A.A. is supported by US National Institutes of Health grant GM31627. S.A., C.I.B. and D.A.A. are supported by the HHMI. J.W., G.M. and C.W. are supported by the Center for Cancer Research, National Cancer Institute and HHMI.",

year = "2013",

month = jan,

doi = "10.1038/NMETH.2277",

language = "English (US)",

volume = "10",

pages = "60--63",

journal = "Nature Methods",

issn = "1548-7091",

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T1 - Fast multicolor 3D imaging using aberration-corrected multifocus microscopy

AU - Abrahamsson, Sara

AU - Chen, Jiji

AU - Hajj, Bassam

AU - Stallinga, Sjoerd

AU - Katsov, Alexander Y.

AU - Wisniewski, Jan

AU - Mizuguchi, Gaku

AU - Soule, Pierre

AU - Mueller, Florian

AU - Darzacq, Claire Dugast

AU - Darzacq, Xavier

AU - Wu, Carl

AU - Bargmann, Cornelia I.

AU - Agard, David A.

AU - Dahan, Maxime

AU - Gustafsson, Mats G.L.

N1 - Funding Information: ensuring continued funding for this project during and after the difficult time of M.G.’s sickness. A.Y.K. is supported by a Jane Coffin Childs postdoctoral fellowship. M.D. acknowledges the support of a Fulbright fellowship. X.D. and M.D. are supported by grant ANR-08-PCVI-0013 from Agence Nationale pour la Recherche. D.A.A. is supported by US National Institutes of Health grant GM31627. S.A., C.I.B. and D.A.A. are supported by the HHMI. J.W., G.M. and C.W. are supported by the Center for Cancer Research, National Cancer Institute and HHMI.

PY - 2013/1

Y1 - 2013/1

N2 - Conventional acquisition of three-dimensional (3D) microscopy data requires sequential z scanning and is often too slow to capture biological events. We report an aberration-corrected multifocus microscopy method capable of producing an instant focal stack of nine 2D images. Appended to an epifluorescence microscope, the multifocus system enables high-resolution 3D imaging in multiple colors with single-molecule sensitivity, at speeds limited by the camera readout time of a single image.

AB - Conventional acquisition of three-dimensional (3D) microscopy data requires sequential z scanning and is often too slow to capture biological events. We report an aberration-corrected multifocus microscopy method capable of producing an instant focal stack of nine 2D images. Appended to an epifluorescence microscope, the multifocus system enables high-resolution 3D imaging in multiple colors with single-molecule sensitivity, at speeds limited by the camera readout time of a single image.

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JF - Nature Methods

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Fast multicolor 3D imaging using aberration-corrected multifocus microscopy

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