A human factors approach to healthcare system redesign emphasizes the involvement of multiple stakeholders in the process. This study identifies work system factors facilitating and hindering the collaboration of multiple stakeholders in a healthcare system redesign project. We interviewed 10 stakeholders participating in the redesign of the family-centered rounds process in a pediatric hospital, and conducted qualitative content analysis of the interview transcripts using the work system model of Smith and Carayon-Sainfort (1989). Various facilitators and barriers to stakeholder collaboration in healthcare system redesign were identified, such as personal characteristics, team characteristics, organizational structure and culture. Results of this study can be used to improve the process used to redesign healthcare work systems and processes that involve multiple stakeholders.