TY - JOUR
T1 - Extracellular matrix-sarcolemmal surface interconnections
T2 - A quick-freeze deep-etch study
AU - Yung, Rex
AU - Frank, Joy S.
PY - 1986
Y1 - 1986
N2 - Using ultrarapid freezing and deep-etch fracture techniques, the extracellular matrix in unfixed rabbit papillary muscles is seen to consist of an extensive network of collagen, microfibrils, and microthreads. The microfibril-microthread lattice appears to weave around collagen fibers connecting them to each other and to the external lamina of the sarcolemma. The external lamina appears to insert into the bilayer via trabeculae. With 10 min exposure to zero-Ca solution, the external lamina of the myocytes detaches from the membrane surface but is held from complete removal by some remaining trabecular attachments. This detachment of external lamina affords a view for the first time of the surface of the myocardial sarcolemma. Particles of varying sizes (6-13 nm) may represent the external portions of some integral proteins or protein molecules associated with the membrane surface. They can also represent attachment sites of the external lamina. The serious risks for the fibrous network structure representing an artifact caused by precipitation of matrix proteins during deep etching are discussed.
AB - Using ultrarapid freezing and deep-etch fracture techniques, the extracellular matrix in unfixed rabbit papillary muscles is seen to consist of an extensive network of collagen, microfibrils, and microthreads. The microfibril-microthread lattice appears to weave around collagen fibers connecting them to each other and to the external lamina of the sarcolemma. The external lamina appears to insert into the bilayer via trabeculae. With 10 min exposure to zero-Ca solution, the external lamina of the myocytes detaches from the membrane surface but is held from complete removal by some remaining trabecular attachments. This detachment of external lamina affords a view for the first time of the surface of the myocardial sarcolemma. Particles of varying sizes (6-13 nm) may represent the external portions of some integral proteins or protein molecules associated with the membrane surface. They can also represent attachment sites of the external lamina. The serious risks for the fibrous network structure representing an artifact caused by precipitation of matrix proteins during deep etching are discussed.
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U2 - 10.1016/0889-1605(86)90017-0
DO - 10.1016/0889-1605(86)90017-0
M3 - Article
C2 - 3681019
AN - SCOPUS:0022740752
SN - 0889-1605
VL - 96
SP - 160
EP - 171
JO - Journal of Ultrastructure Research and Molecular Structure Research
JF - Journal of Ultrastructure Research and Molecular Structure Research
IS - 1-3
ER -