TY - JOUR
T1 - Extensive and Selective Mutation of a Rearranged VH5 Gene in Human B Cell Chronic Lymphocytic Leukemia
AU - Cai, Jilian
AU - Humphries, Caroline
AU - Richardson, Andrea
AU - Tucker, Philip W.
PY - 1992/10/1
Y1 - 1992/10/1
N2 - B cell chronic lymphocytic leukemia (CLL) is the malignant, monoclonal equivalent of a human CD5+ B cell. Previous studies have shown that the VH and VL genes rearranged and/or expressed in CLL have few and apparently random mutations. However, in this study, we have found that the rearranged VH251 gene, one of the three-membered VH5 family, has extensive and selective mutations in B-CLL cells. Somatic mutation at the nudeotide level is 6.03% in B-CLLs whereas the somatic mutation levels are much lower in CD5+ and CD5− cord B cells, adult peripheral blood B cells, and Epstein-Barr virus-transformed CD5H B cell lines (0.45, 0.93, and 1.92%, respectively). Complementary determining region I (CDR1) mutation in CLLs is particularly prevalent, and interchanges in CDRs often lead to acquisition of charge. Analysis of somatic mutations and mutations to charged residues demonstrated that the mutations in CLLs are highly selected.
AB - B cell chronic lymphocytic leukemia (CLL) is the malignant, monoclonal equivalent of a human CD5+ B cell. Previous studies have shown that the VH and VL genes rearranged and/or expressed in CLL have few and apparently random mutations. However, in this study, we have found that the rearranged VH251 gene, one of the three-membered VH5 family, has extensive and selective mutations in B-CLL cells. Somatic mutation at the nudeotide level is 6.03% in B-CLLs whereas the somatic mutation levels are much lower in CD5+ and CD5− cord B cells, adult peripheral blood B cells, and Epstein-Barr virus-transformed CD5H B cell lines (0.45, 0.93, and 1.92%, respectively). Complementary determining region I (CDR1) mutation in CLLs is particularly prevalent, and interchanges in CDRs often lead to acquisition of charge. Analysis of somatic mutations and mutations to charged residues demonstrated that the mutations in CLLs are highly selected.
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U2 - 10.1084/jem.176.4.1073
DO - 10.1084/jem.176.4.1073
M3 - Article
C2 - 1402653
AN - SCOPUS:0026759657
SN - 0022-1007
VL - 176
SP - 1073
EP - 1081
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
IS - 4
ER -