Expression of the CB1 cannabinoid receptor in macrophage-like cells from brain tissue: Immunochemical characterization by fusion protein antibodies

Debasish Sinha; Tom I. Bonner; Narayan R. Bhat; Lisa A. Matsuda

doi:10.1016/S0165-5728(97)00181-1

Expression of the CB1 cannabinoid receptor in macrophage-like cells from brain tissue: Immunochemical characterization by fusion protein antibodies

Debasish Sinha, Tom I. Bonner, Narayan R. Bhat, Lisa A. Matsuda

Research output: Contribution to journal › Article › peer-review

54 Scopus citations

Abstract

Antibodies designed to recognize a 74 amino acid sequence of the N- or C-terminal domain of the rat CB1 cannabinoid receptor detected a 58 kDa protein in immunoblots of brain and various cells known to express the CB1 cannabinoid receptor. A human B-lymphoblastoid cell line and macrophage-like cells from neonatal rat brain were also positive for CB1 receptor-like immunoreactivity. Immunocytochemical analysis performed with isolated Fab fragments showed surface staining in NG108-15 cells and brain macrophage like cells which also express MHC class II antigens. The data suggest a plausible role for CB1 receptors in the immune function of brain.

Original language	English (US)
Pages (from-to)	13-21
Number of pages	9
Journal	Journal of Neuroimmunology
Volume	82
Issue number	1
DOIs	https://doi.org/10.1016/S0165-5728(97)00181-1
State	Published - Feb 1998
Externally published	Yes

Keywords

Cannabis
G protein coupled receptors
Marijuana
Microglia

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Neurology
Clinical Neurology

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10.1016/S0165-5728(97)00181-1

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title = "Expression of the CB1 cannabinoid receptor in macrophage-like cells from brain tissue: Immunochemical characterization by fusion protein antibodies",

abstract = "Antibodies designed to recognize a 74 amino acid sequence of the N- or C-terminal domain of the rat CB1 cannabinoid receptor detected a 58 kDa protein in immunoblots of brain and various cells known to express the CB1 cannabinoid receptor. A human B-lymphoblastoid cell line and macrophage-like cells from neonatal rat brain were also positive for CB1 receptor-like immunoreactivity. Immunocytochemical analysis performed with isolated Fab fragments showed surface staining in NG108-15 cells and brain macrophage like cells which also express MHC class II antigens. The data suggest a plausible role for CB1 receptors in the immune function of brain.",

keywords = "Cannabis, G protein coupled receptors, Marijuana, Microglia",

author = "Debasish Sinha and Bonner, {Tom I.} and Bhat, {Narayan R.} and Matsuda, {Lisa A.}",

note = "Funding Information: We thank Dr. Robert P. Thompson for helping us with confocal microscopy, Mr. Gregory Miller for technical assistance and Dr. Graeme Wistow for his help and support during the preparation of this manuscript. This research was supported by DA 08104 and the Medical University of South Carolina Institutional Research Funds of 1992-1993 to L.A.M.",

year = "1998",

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doi = "10.1016/S0165-5728(97)00181-1",

language = "English (US)",

volume = "82",

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journal = "Journal of Neuroimmunology",

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T2 - Immunochemical characterization by fusion protein antibodies

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AU - Bonner, Tom I.

AU - Bhat, Narayan R.

AU - Matsuda, Lisa A.

N1 - Funding Information: We thank Dr. Robert P. Thompson for helping us with confocal microscopy, Mr. Gregory Miller for technical assistance and Dr. Graeme Wistow for his help and support during the preparation of this manuscript. This research was supported by DA 08104 and the Medical University of South Carolina Institutional Research Funds of 1992-1993 to L.A.M.

PY - 1998/2

Y1 - 1998/2

N2 - Antibodies designed to recognize a 74 amino acid sequence of the N- or C-terminal domain of the rat CB1 cannabinoid receptor detected a 58 kDa protein in immunoblots of brain and various cells known to express the CB1 cannabinoid receptor. A human B-lymphoblastoid cell line and macrophage-like cells from neonatal rat brain were also positive for CB1 receptor-like immunoreactivity. Immunocytochemical analysis performed with isolated Fab fragments showed surface staining in NG108-15 cells and brain macrophage like cells which also express MHC class II antigens. The data suggest a plausible role for CB1 receptors in the immune function of brain.

AB - Antibodies designed to recognize a 74 amino acid sequence of the N- or C-terminal domain of the rat CB1 cannabinoid receptor detected a 58 kDa protein in immunoblots of brain and various cells known to express the CB1 cannabinoid receptor. A human B-lymphoblastoid cell line and macrophage-like cells from neonatal rat brain were also positive for CB1 receptor-like immunoreactivity. Immunocytochemical analysis performed with isolated Fab fragments showed surface staining in NG108-15 cells and brain macrophage like cells which also express MHC class II antigens. The data suggest a plausible role for CB1 receptors in the immune function of brain.

KW - Cannabis

KW - G protein coupled receptors

KW - Marijuana

KW - Microglia

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Expression of the CB1 cannabinoid receptor in macrophage-like cells from brain tissue: Immunochemical characterization by fusion protein antibodies

Abstract

Keywords

ASJC Scopus subject areas

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