TY - JOUR
T1 - Expression of Prostate-specific membrane antigen in transitional cell carcinoma of the bladder
T2 - Prognostic value?
AU - Gala, Jean Luc
AU - Loric, Sylvain
AU - Guiot, Yves
AU - Denmeade, Samuel Ray
AU - Gady, Alyssa
AU - Brasseur, Francis
AU - Heusterspreute, Michel
AU - Eschwege, Pascal
AU - De Nayer, Philippe
AU - Van Cangh, Paul
AU - Tombal, Bertrand
PY - 2000/1/1
Y1 - 2000/1/1
N2 - The expression of Prostate-specific membrane antigen (PSMA) mRNA was assessed in the normal bladder urothelium (n = 9), transitional cell carcinoma (TCC) specimens (n = 52), TCC-derived cell lines (n = 3), and preoperative blood samples from TCC patients (n = 27), Specific PSMA mRNA was found in 100% of normal and malignant tissues and two cell lines. PSMA protein was detected in normal (n = 3) and malignant tissues (n = 4). Using a PSMA-specific substrate, PSMA enzymatic activity was found in two bladder cell lines and correlated with immunostaining. Seven of the 27 TCC preoperative blood samples were positive by reverse transcription-PCR. These preliminary results, obtained on a nonrandomized cohort of patients, correlated with tumor invasion (positive RT-PCR: 0% for pT ≤2 versus 4.1% for pT ≥3) and 2-year survival rate (81% in the PSMA-negative group versus 29% in the PSMA-positive group). Although the clinical usefulness of this assay requires confirmation in larger prospective randomized trials, current preliminary results suggest that a blood-borne PSMA mRNA PCR assay may be a useful tool to predict a poor outcome in TCC patients.
AB - The expression of Prostate-specific membrane antigen (PSMA) mRNA was assessed in the normal bladder urothelium (n = 9), transitional cell carcinoma (TCC) specimens (n = 52), TCC-derived cell lines (n = 3), and preoperative blood samples from TCC patients (n = 27), Specific PSMA mRNA was found in 100% of normal and malignant tissues and two cell lines. PSMA protein was detected in normal (n = 3) and malignant tissues (n = 4). Using a PSMA-specific substrate, PSMA enzymatic activity was found in two bladder cell lines and correlated with immunostaining. Seven of the 27 TCC preoperative blood samples were positive by reverse transcription-PCR. These preliminary results, obtained on a nonrandomized cohort of patients, correlated with tumor invasion (positive RT-PCR: 0% for pT ≤2 versus 4.1% for pT ≥3) and 2-year survival rate (81% in the PSMA-negative group versus 29% in the PSMA-positive group). Although the clinical usefulness of this assay requires confirmation in larger prospective randomized trials, current preliminary results suggest that a blood-borne PSMA mRNA PCR assay may be a useful tool to predict a poor outcome in TCC patients.
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M3 - Article
C2 - 11051255
AN - SCOPUS:0033765577
SN - 1078-0432
VL - 6
SP - 4049
EP - 4054
JO - Clinical Cancer Research
JF - Clinical Cancer Research
IS - 10
ER -