TY - JOUR
T1 - Expression of key regulators of mitochondrial biogenesis in Growth Hormone Receptor Knockout (GHRKO) mice is enhanced but is not further improved by other potential life-extending interventions
AU - Gesing, Adam
AU - Masternak, Michal M.
AU - Wang, Feiya
AU - Joseph, Anna Maria
AU - Leeuwenburgh, Christiaan
AU - Westbrook, Reyhan
AU - Lewinski, Andrzej
AU - Karbownik-Lewinska, Malgorzata
AU - Bartke, Andrzej
N1 - Funding Information:
The present study was supported by National Institute on Aging (AG 19899, U19 AG023122, AG31736, and AG032290), the Ellison Medical Foundation, Southern Illinois University School of Medicine, Claude D. Pepper Older Americans Independence Center (1P30AG028740), and Polish Ministry of Science and Higher Education (N N401 042638).
PY - 2011/10
Y1 - 2011/10
N2 - Mitochondrial biogenesis is essential for cell viability. Growth hormone receptor knockout (GHRKO), calorie restriction, and surgical visceral fat removal constitute experimental interventions to delay aging and increase life span. We examined the expression of known regulators of mitochondriogenesis: peroxisome proliferator-activated receptor γ co-activator 1α (PGC-1α), adenosine monophosphate (AMP)-activated protein kinase (AMPK), sirtuin-1 (SIRT-1) and sirtuin-3 (SIRT-3), endothelial nitric oxide synthase (eNOS), nuclear respiratory factor-1, mitochondrial transcription factor A (TFAM), and mitofusin-2 (MFN-2) in the skeletal muscles and hearts of control and calorie-restricted female GHRKO mice and in the kidneys of male GHRKOs after visceral fat removal or sham surgery. Expression of PGC-1α in skeletal muscles, AMPK, SIRT-1, SIRT-3, eNOS, and MFN-2 in the heart and PGC-1α, AMPK, SIRT-3, eNOS, and MFN-2 in kidneys was increased in GHRKO mice but was not affected by calorie restriction or visceral fat removal. GHRKO mice have increased expression of key regulators of mitochondriogenesis, which is not improved further by calorie restriction or visceral fat removal.
AB - Mitochondrial biogenesis is essential for cell viability. Growth hormone receptor knockout (GHRKO), calorie restriction, and surgical visceral fat removal constitute experimental interventions to delay aging and increase life span. We examined the expression of known regulators of mitochondriogenesis: peroxisome proliferator-activated receptor γ co-activator 1α (PGC-1α), adenosine monophosphate (AMP)-activated protein kinase (AMPK), sirtuin-1 (SIRT-1) and sirtuin-3 (SIRT-3), endothelial nitric oxide synthase (eNOS), nuclear respiratory factor-1, mitochondrial transcription factor A (TFAM), and mitofusin-2 (MFN-2) in the skeletal muscles and hearts of control and calorie-restricted female GHRKO mice and in the kidneys of male GHRKOs after visceral fat removal or sham surgery. Expression of PGC-1α in skeletal muscles, AMPK, SIRT-1, SIRT-3, eNOS, and MFN-2 in the heart and PGC-1α, AMPK, SIRT-3, eNOS, and MFN-2 in kidneys was increased in GHRKO mice but was not affected by calorie restriction or visceral fat removal. GHRKO mice have increased expression of key regulators of mitochondriogenesis, which is not improved further by calorie restriction or visceral fat removal.
KW - Calorie restriction
KW - GHRKO mice
KW - Gene expression
KW - Mitochondrial biogenesis
KW - Visceral fat removal
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U2 - 10.1093/gerona/glr080
DO - 10.1093/gerona/glr080
M3 - Article
C2 - 21788651
AN - SCOPUS:80052912001
SN - 1079-5006
VL - 66 A
SP - 1062
EP - 1076
JO - Journals of Gerontology - Series A Biological Sciences and Medical Sciences
JF - Journals of Gerontology - Series A Biological Sciences and Medical Sciences
IS - 10
ER -