Expression of agrin in the developing and adult rat brain

N. A. Cohen, W. E. Kaufmann, P. F. Worley, F. Rupp

Research output: Contribution to journalArticlepeer-review

64 Scopus citations


Agrin, a synaptic basal lamina protein, is essential for the formation of the vertebrate neuromuscular junction. Agrin's role in synaptogenesis in the central nervous system has, however, not been elucidated. Therefore, we performed immunohistochemical analysis of agrin localization in adult rat brain using agrin-specific polyclonal antibodies. Our results show that agrin immunoreactivity is detected in neuronal cells throughout the brain, and that agrin is expressed in many morphologically and neurochemically distinct neuronal populations. Within neurons, agrin-immunoreactive material is present in dendrites. To determine agrin isoform expression in the central nervous system, we analysed the pattern of expression of several isoforms during development of the rat brain. Our results indicate that alternative splicing of agrin is specifically regulated in the nervous system; isoforms of the Y=4 (i.e. Ag x,4,0, Ag x,4,8 and Ag x,4,19), Z=8 and Z=19 type are expressed exclusively in the nervous system. Agrin expression precedes synaptogenesis and is developmentally regulated in neural tissues. To evaluate stimuli that may be involved in the regulation of agrin expression, we monitored the patterns of isoform expression following a depolarizing stimulus. Our results show that agrin expression in the adult hippocampus is regulated in an activity-dependent manner, with kinetics of induction resembling a delayed early response gene.

Original languageEnglish (US)
Pages (from-to)581-596
Number of pages16
Issue number2
StatePublished - Jan 1997


  • Activity dependent
  • Agrin immunoreactivity
  • Agrin isoforms
  • Alternative splicing
  • Development
  • In situ hybridization

ASJC Scopus subject areas

  • General Neuroscience


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