TY - JOUR
T1 - Expression and regulation of chicken fibroblast growth factor homologous factor (FHF)-4 during craniofacial morphogenesis
AU - Muñoz-Sanjuán, Ignacio
AU - Cooper, Michael K.
AU - Beachy, Philip A.
AU - Fallon, John F.
AU - Nathans, Jeremy
PY - 2001
Y1 - 2001
N2 - Fibroblast growth factor homologous factors (FHFs) have been implicated in limb and nervous system development. In this paper we describe the expression of the cFHF-4 gene during chicken craniofacial development. cFHF-4 is expressed in the mesenchyme of the frontonasal process, and in the mesenchyme and ectoderm of the mandibular processes. The expression of cFHF-4 and other genes implicated in facial patterning have been analyzed in talpid2 embryos or in the presence of exogenous retinoic acid. Talpid2 mutants show abnormal patterns of gene expression, including up-regulation of cFHF-4 in the developing face, which correlate with defects in cartilage formation. By contrast, expression of cFHF-4 in the developing face is strongly downregulated by teratogenic doses of all-trans retinoic acid in a dose-dependent manner. Low levels of retinoic acid that produce distal upper beak truncations do not affect cShh, c-Patched-1, or c-Bmp-2 expression in the face, but downregulate cFHF-4 in the frontonasal process.
AB - Fibroblast growth factor homologous factors (FHFs) have been implicated in limb and nervous system development. In this paper we describe the expression of the cFHF-4 gene during chicken craniofacial development. cFHF-4 is expressed in the mesenchyme of the frontonasal process, and in the mesenchyme and ectoderm of the mandibular processes. The expression of cFHF-4 and other genes implicated in facial patterning have been analyzed in talpid2 embryos or in the presence of exogenous retinoic acid. Talpid2 mutants show abnormal patterns of gene expression, including up-regulation of cFHF-4 in the developing face, which correlate with defects in cartilage formation. By contrast, expression of cFHF-4 in the developing face is strongly downregulated by teratogenic doses of all-trans retinoic acid in a dose-dependent manner. Low levels of retinoic acid that produce distal upper beak truncations do not affect cShh, c-Patched-1, or c-Bmp-2 expression in the face, but downregulate cFHF-4 in the frontonasal process.
KW - Craniofacial development
KW - Retinoic acid
KW - Talpid
UR - http://www.scopus.com/inward/record.url?scp=0035109018&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035109018&partnerID=8YFLogxK
U2 - 10.1002/1097-0177(20010301)220:3<238::AID-DVDY1104>3.0.CO;2-T
DO - 10.1002/1097-0177(20010301)220:3<238::AID-DVDY1104>3.0.CO;2-T
M3 - Article
C2 - 11241832
AN - SCOPUS:0035109018
SN - 1058-8388
VL - 220
SP - 238
EP - 245
JO - Developmental Dynamics
JF - Developmental Dynamics
IS - 3
ER -