Expression and localization of MrgprD in mouse intestinal tract

Chenxing Zhou, Jia Li, Lin Liu, Zongxiang Tang, Fengyi Wan, Lei Lan

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


MrgprD, a Mas-related G protein-coupled receptor, is initially identified in sensory neurons of mouse dorsal root ganglia (DRG) and has been suggested to participate in somatosensation. However, MrgprD has recently been found to be expressed outside the nervous system such as in aortic endothelia cells and neutrophils. In this study, we used immunohistochemistry to detect the expression and localization of MrgprD in mouse intestinal tract. The immunoreactivity (IR) of MrgprD was found in the smooth muscle layers of small intestine, colon and rectum. In addition, MrgprD IR was colocalized with F4/80-positive macrophages and CD3-positive T lymphocytes resident in the lamina propria of intestinal mucosa. MrgprD was also found to be expressed in primary peritoneal macrophages and splenic T lymphocytes. Furthermore, the presence of MrgprD mRNA and its protein was detected in murine macrophage-like RAW 264.7 and human T lymphocyte Jurkat cell lines. Our study shows, for the first time, the expression and localization of MrgprD in the intestinal tract and in macrophages and T lymphocytes, indicating the potential roles of MrgprD in intestinal mobility and immunity.

Original languageEnglish (US)
Pages (from-to)259-268
Number of pages10
JournalCell and Tissue Research
Issue number2
StatePublished - Aug 14 2019


  • Intestinal tract
  • Macrophages
  • MrgprD
  • Smooth muscle cells
  • T lymphocytes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology


Dive into the research topics of 'Expression and localization of MrgprD in mouse intestinal tract'. Together they form a unique fingerprint.

Cite this