Expression and function of a recombinant PDGF B gene in porcine arteries

Vincent J. Pompili; David Gordon; Hong San; Zhiyong Yang; David W M Muller; Gary J. Nabel; Elizabeth G. Nabel

Expression and function of a recombinant PDGF B gene in porcine arteries

Vincent J. Pompili, David Gordon, Hong San, Zhiyong Yang, David W M Muller, Gary J. Nabel, Elizabeth G. Nabel

Research output: Contribution to journal › Article › peer-review

Abstract

Platelet derived growth factor (PDGF) B is a mitogen and chemoattractant for smooth muscle cells in vitro, and expression of a recombinant PDGF B gene in porcine arteries stimulates intimal thickening. To define the mechanisms by which PDGF B gene expression induces intimal thickening in vivo, we examined its effects on smooth muscle cell proliferation and migration, extracellular matrix synthesis, and inflammatory cell infiltration in intimal lesions of pig arteries after direct gene transfer of a recombinant PDGF B gone. PDGF B gent expression was associated with rapid formation of an intima, including 3- to 10-fold increases in intimal thickness and intima- to-media area ratio 4 to 21 days after gene transfer compared with control transfected arteries. Intimal smooth muscle cell proliferation was detected at 2 days, peaked at 7 days (P

Original language	English (US)
Pages (from-to)	2254-2264
Number of pages	11
Journal	Arteriosclerosis, Thrombosis, and Vascular Biology
Volume	15
Issue number	12
State	Published - Dec 1995
Externally published	Yes

Keywords

cell proliferation
gene expression
gene transfer
neointima
PDGF B gene

ASJC Scopus subject areas

Cardiology and Cardiovascular Medicine

Cite this

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abstract = "Platelet derived growth factor (PDGF) B is a mitogen and chemoattractant for smooth muscle cells in vitro, and expression of a recombinant PDGF B gene in porcine arteries stimulates intimal thickening. To define the mechanisms by which PDGF B gene expression induces intimal thickening in vivo, we examined its effects on smooth muscle cell proliferation and migration, extracellular matrix synthesis, and inflammatory cell infiltration in intimal lesions of pig arteries after direct gene transfer of a recombinant PDGF B gone. PDGF B gent expression was associated with rapid formation of an intima, including 3- to 10-fold increases in intimal thickness and intima- to-media area ratio 4 to 21 days after gene transfer compared with control transfected arteries. Intimal smooth muscle cell proliferation was detected at 2 days, peaked at 7 days (P",

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author = "Pompili, {Vincent J.} and David Gordon and Hong San and Zhiyong Yang and Muller, {David W M} and Nabel, {Gary J.} and Nabel, {Elizabeth G.}",

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T1 - Expression and function of a recombinant PDGF B gene in porcine arteries

AU - Pompili, Vincent J.

AU - Gordon, David

AU - San, Hong

AU - Yang, Zhiyong

AU - Muller, David W M

AU - Nabel, Gary J.

AU - Nabel, Elizabeth G.

PY - 1995/12

Y1 - 1995/12

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AB - Platelet derived growth factor (PDGF) B is a mitogen and chemoattractant for smooth muscle cells in vitro, and expression of a recombinant PDGF B gene in porcine arteries stimulates intimal thickening. To define the mechanisms by which PDGF B gene expression induces intimal thickening in vivo, we examined its effects on smooth muscle cell proliferation and migration, extracellular matrix synthesis, and inflammatory cell infiltration in intimal lesions of pig arteries after direct gene transfer of a recombinant PDGF B gone. PDGF B gent expression was associated with rapid formation of an intima, including 3- to 10-fold increases in intimal thickness and intima- to-media area ratio 4 to 21 days after gene transfer compared with control transfected arteries. Intimal smooth muscle cell proliferation was detected at 2 days, peaked at 7 days (P

KW - cell proliferation

KW - gene expression

KW - gene transfer

KW - neointima

KW - PDGF B gene

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Expression and function of a recombinant PDGF B gene in porcine arteries

Abstract

Keywords

ASJC Scopus subject areas

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