Experimental analysis of the mechanism of chromatin remodeling by rna polymerase ii

Daria A. Gaykalova, Olga I. Kulaeva, Nikolai A. Pestov, Fu Kai Hsieh, Vasily M. Studitsky

Research output: Chapter in Book/Report/Conference proceedingChapter

13 Scopus citations


The vital process of transcription by RNA polymerase II (Pol II) occurs in chromatin environment in eukaryotic cells; in fact, moderately transcribed genes retain nucleosomal structure. Recent studies suggest that chromatin structure presents a strong barrier for transcribing Pol II in vitro, and that DNA-histone interactions are only partially and transiently disrupted during transcript elongation on moderately active genes. Furthermore, elongating Pol II complex is one of the major targets during gene regulation. Below, we describe a highly purified, defined experimental system that recapitulates many important properties of transcribed chromatin in vitro and allows detailed analysis of the underlying mechanisms.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Number of pages22
StatePublished - 2012
Externally publishedYes

Publication series

NameMethods in Enzymology
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988


  • DNA uncoiling
  • Histone octamer
  • Nucleosome
  • Structures
  • Transcript elongation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology


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