Exogenous nucleic acids aggregate in non-P-body cytoplasmic granules when transfected into cultured cells

Huang Huang, Na Wei, Yingfei Xiong, Feng Yang, Huaqiang Fang, Wenjun Xie, Zhuan Zhou, Heping Cheng, Zicai Liang, Quan Du

Research output: Contribution to journalArticlepeer-review


To modulate gene expression in research studies or in potential clinical therapies, transfection of exogenous nucleic acids including plasmid DNA and small interference RNA (siRNA) are generally performed. However, the cellular processing and the fate of these nucleic acids remain elusive. By investigating the cellular behavior of transfected nucleic acids using confocal imaging, here we show that when siRNA was co-transfected into cultured cells with other nucleic acids, including single-stranded RNA oligonucleotides, single and double-stranded DNA oligonucleotides, as well as long double-stranded plasmid DNA, they all aggregate in the same cytoplasmic granules. Interestingly, the amount of siRNA aggregating in granules was found not to correlate with the gene silencing activity, suggesting that assembly of cytoplasmic granules triggered by siRNA transfection may be separable from the siRNA silencing event. Our results argue against the claim that the siRNA-aggregating granules are the functional site of RNA interference (RNAi). Taken together, our studies suggest that, independent of their types or forms, extraneously transfected nucleic acids are processed through a common cytoplasmic pathway and trigger the formation of a new type of cytoplasmic granules "transfection granules".

Original languageEnglish (US)
Pages (from-to)272-281
Number of pages10
JournalFrontiers of Biology in China
Issue number3
StatePublished - Jun 2010
Externally publishedYes


  • Nucleic acids
  • P-body
  • RNA interference (RNAi)
  • Small interference RNA (siRNA)
  • Transfection

ASJC Scopus subject areas

  • Biotechnology
  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Genetics


Dive into the research topics of 'Exogenous nucleic acids aggregate in non-P-body cytoplasmic granules when transfected into cultured cells'. Together they form a unique fingerprint.

Cite this