TY - JOUR
T1 - Exogenous ATP and other nucleoside phosphates modulate epidermal growth factor receptors of A-431 epidermoid carcinoma cells
AU - Hosoi, K.
AU - Edidin, M.
PY - 1989
Y1 - 1989
N2 - The binding of epidermal growth factor (EGF) by A-431 human epidermoid carcinoma cells was reduced after exposure of the cells to low concentrations (0.01-1 mM) of ATP and other nucleoside 5'-triphosphates at 37°C, but not at 0°C. This was due to loss of high-affinity EGF binding sites. The modulation was associated with transient increases in inositol phosphate synthesis and intracellular Ca2+ and with phosphorylation of the EGF receptor on serine and threonine. There was no evidence for entry of labeled ATP into the cells. ATP appeared to bind to specific cell surface receptors. Such binding was demonstrated directly with the nonmetabolizable ATP analogue adenosine 5'-[β,γ-imido]triphosphate.
AB - The binding of epidermal growth factor (EGF) by A-431 human epidermoid carcinoma cells was reduced after exposure of the cells to low concentrations (0.01-1 mM) of ATP and other nucleoside 5'-triphosphates at 37°C, but not at 0°C. This was due to loss of high-affinity EGF binding sites. The modulation was associated with transient increases in inositol phosphate synthesis and intracellular Ca2+ and with phosphorylation of the EGF receptor on serine and threonine. There was no evidence for entry of labeled ATP into the cells. ATP appeared to bind to specific cell surface receptors. Such binding was demonstrated directly with the nonmetabolizable ATP analogue adenosine 5'-[β,γ-imido]triphosphate.
UR - http://www.scopus.com/inward/record.url?scp=0024351766&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024351766&partnerID=8YFLogxK
U2 - 10.1073/pnas.86.12.4510
DO - 10.1073/pnas.86.12.4510
M3 - Article
C2 - 2786633
AN - SCOPUS:0024351766
SN - 0027-8424
VL - 86
SP - 4510
EP - 4514
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 12
ER -