Exercise training reduces PGE2 levels and induces recovery from steatosis in tumor-bearing rats

F. S. Lira, A. S. Yamashita, L. C. Carnevali, D. C. Gonçalves, W. P. Lima, J. C. Rosa, E. C. Caperuto, L. F.C. Rosa, M. Seelaender

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

The effects of endurance training on PGE2 levels and upon the maximal activity of hepatic carnitine palmitoyltransferase (CPT) system were studied in rats bearing the Walker 256 carciosarcoma. Animals were randomly assigned to a sedentary control (SC), sedentary tumor-bearing (ST), exercised control (EC), and as an exercised tumor-bearing (ET) group. Trained rats ran on a treadmill (60% VO2 max) for 60min/day, 5 days/week, for 8 weeks. We examined the mRNA expression (RT-PCR) and maximal activity (radioassay) of the carnitine palmitoyltransferase system enzymes (CPT I and CPT II), as well as the gene expression of fatty-acid-binding protein (L-FABP) in the liver. PGE 2 content was measured in the serum, in tumor cells, and in the liver (ELISA). CPT I and CPT II maximal activity were decreased (p<0.01) in ST when compared with SC. In contrast, serum PGE2 was increased (p<0.05) in cachectic animals as compared with SC. In the liver, PGE 2 content was also increased (p<0.05) when compared with SC. Endurance training restored maximal CPT I and CPT II activity in the tumor-bearing animals (p<0.0001). Exercise training induced PGE2 levels to return to control values in the liver of tumor-bearing training rats (p<0.05) and decreased the eicosanoid content in the tumor (p<0.01). In conclusion, endurance training was capable of reestablishing liver carnitine palmitoyltransferase (CPT) system activity associated with decreased PGE 2 levels in cachectic tumor-bearing animals, preventing steatosis.

Original languageEnglish (US)
Pages (from-to)944-949
Number of pages6
JournalHormone and Metabolic Research
Volume42
Issue number13
DOIs
StatePublished - 2010
Externally publishedYes

Keywords

  • CPT system
  • PGE levels
  • cachexia
  • exercise training

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

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