Evidence for the production of a nonsteroidal endotheliotropic factor by human granulosa cells in culture

E. Katz, A. L. Goodman, J. Garcia, J. D. Rone

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Objective: To determine the cellular source of the angiogenic activity displayed by follicular fluid (FF). Design: Human granulosa cells were harvested from 27 follicular aspirates obtained 34 to 36 hours after eight patients, previously treated with human menopausal gonadotropin (hMG), follicle-stimulating hormone plus hMG, or clomiphene citrate and hMG received human chorionic gonadotropin (10,000 IU intramuscularly). Granulosa cells from individual follicles were plated at 50,000 cells/cm2 in Medium 199 (Sigma, St. Louis, MO) supplemented with either 5% calf serum or 0.1% bovine serum albumin; media collected 24 hours later was assayed in vitro measuring endothelial cell migration. Fractions depleted of steroids by reversed phase C1 chromatography were assayed as well. Results: Granulosa cell-conditioned media from 18 of 27 follicles significantly stimulated endothelial cell migration (P < 0.05). Chemoattractant activity did not appear to be related to steroid accumulation in the media and was not diminished in steroid depleted fractions. Conclusions: These findings suggest that human granulosa cells are a source of (nonsteroidal) endotheliotropic-angiogenic activity in FF.

Original languageEnglish (US)
Pages (from-to)107-112
Number of pages6
JournalFertility and sterility
Issue number1
StatePublished - 1992
Externally publishedYes


  • Angiogenesis
  • folliculogenesis
  • granulosa cells

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology


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