Evaluation of the CHIRON®RIBA™HSV type -1/type-2 strip immunoblot assay (SIA) for typing of HSV antibodies (Ab)

R. Ashley, A. Warford, C. Cleaves, J. Zenilman, D. Alexander, L. Corey

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Objective: Evaluate the RIBA™ HSV 1/2 SU for HSV Ab typing in culture documented HSV-1 and HSV-2 cases, with the CDC HSV and CMV Evaluation Panel (CDC-EP), and in selected clinical populations. Methods: Sera from 27 HSV-1 and 339 HSV-2 culture positive subjects were tested by RIBA™ SIA. A second study compared RIBA™ SIA against the CDC-EP, which consists of 100 well characterized sera from healthy donors. Results from the study were compared to those from the CDC-EIA and the CDC-gG blot for Ab to HSV-1 and HSV-2. In addition, 858 specimens from STD clinic clients, children, pregnant women, and transplant patients were tested by the RIBA™ SIA and a commercial HSV EIA with Ab typing confirmed by Western Blot (UW). Results: Against culture, sensitivity of the RIBA™ SIA was 93% for HSV-1 and 99% for HSV-2. In the second study with the CDC-EP, RIBA™ SIA showed 100% sensitivity and specificity vs CDC-EIA, with a 97% typing agreement with the CDC-gG blot for HSV-1 and HSV-2. In the 858 sera from different patient populations, sensitivity and specificity of the RIBA™ SIA vs EIA were 95% and 99% respectively with PPVs of 97% for HSV-1 and HSV-2 vs Western Blot. Conclusion: RIBA™ SIA provides accurate HSV Ab typing results compared to culture and Western Blot. This accurate, easy to use, type specific serological test may have wide clinical applicability.

Original languageEnglish (US)
Number of pages1
JournalClinical Infectious Diseases
Issue number2
StatePublished - Dec 1 1997

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases


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